基于细菌的重金属生物吸附剂的开发：表达金属结合基序的大肠杆菌对镉和汞的摄取增强
Development of bacterium-based heavy metal biosorbents: enhanced uptake of cadmium and mercury by Escherichia coli expressing a metal binding motif.
作者信息
Pazirandeh M, Wells B M, Ryan R L
机构信息
Center for Bio/Molecular Science and Engineering, Naval Research Laboratory, Washington, D.C. 20375, USA.
出版信息
Appl Environ Microbiol. 1998 Oct;64(10):4068-72. doi: 10.1128/AEM.64.10.4068-4072.1998.
Abstract
A gene coding for a de novo peptide sequence containing a metal binding motif was chemically synthesized and expressed in Escherichia coli as a fusion with the maltose binding protein. Bacterial cells expressing the metal binding peptide fusion demonstrated enhanced binding of Cd2+ and Hg2+ compared to bacterial cells lacking the metal binding peptide. The potential use of genetically engineered bacteria as biosorbents for the removal of heavy metals from wastewaters is discussed.
摘要
一个编码含有金属结合基序的从头合成肽序列的基因被化学合成，并在大肠杆菌中作为与麦芽糖结合蛋白的融合体进行表达。与缺乏金属结合肽的细菌细胞相比，表达金属结合肽融合体的细菌细胞对Cd2+和Hg2+的结合能力增强。本文讨论了利用基因工程细菌作为生物吸附剂从废水中去除重金属的潜在用途。
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本文引用的文献
1
Enhanced metalloadsorption of bacterial cells displaying poly-His peptides.展示多组氨酸肽的细菌细胞对金属的吸附增强。
Nat Biotechnol. 1996 Aug;14(8):1017-20. doi: 10.1038/nbt0896-1017.
2
Metalloadsorption by Escherichia coli cells displaying yeast and mammalian metallothioneins anchored to the outer membrane protein LamB.通过展示锚定在外膜蛋白LamB上的酵母和哺乳动物金属硫蛋白的大肠杆菌细胞进行金属吸附。
J Bacteriol. 1998 May;180(9):2280-4. doi: 10.1128/JB.180.9.2280-2284.1998.
3
Engineering metal-binding sites in proteins.在蛋白质中设计金属结合位点。
Curr Opin Struct Biol. 1997 Aug;7(4):495-500. doi: 10.1016/s0959-440x(97)80112-1.
4
Construction and characterization of Escherichia coli genetically engineered for bioremediation of Hg(2+)-contaminated environments.用于汞（2+）污染环境生物修复的基因工程大肠杆菌的构建与表征
Appl Environ Microbiol. 1997 Jun;63(6):2442-5. doi: 10.1128/aem.63.6.2442-2445.1997.
5
Development of a metallothionein based heavy metal biosorbent.一种基于金属硫蛋白的重金属生物吸附剂的研发。
Biochem Mol Biol Int. 1996 Jul;39(4):789-95. doi: 10.1080/15216549600201881.
6
Expression of the Neurospora crassa metallothionein gene in Escherichia coli and its effect on heavy-metal uptake.粗糙脉孢菌金属硫蛋白基因在大肠杆菌中的表达及其对重金属吸收的影响。
Appl Microbiol Biotechnol. 1995 Nov;43(6):1112-7. doi: 10.1007/BF00166934.
7
Lactose fed-batch overexpression of recombinant metalloproteins in Escherichia coli BL21 (DE3): process control yielding high levels of metal-incorporated, soluble protein.在大肠杆菌BL21(DE3)中乳糖补料分批培养重组金属蛋白的过表达：过程控制可产生高水平的结合金属的可溶性蛋白。
Protein Expr Purif. 1995 Oct;6(5):646-54. doi: 10.1006/prep.1995.1085.
8
The prediction and characterization of metal binding sites in proteins.蛋白质中金属结合位点的预测与表征。
Protein Eng. 1993 Jan;6(1):29-35. doi: 10.1093/protein/6.1.29.
9
Characterization of enzymatic reduction of hexavalent chromium by Escherichia coli ATCC 33456.大肠杆菌ATCC 33456对六价铬的酶促还原特性研究。
Appl Environ Microbiol. 1993 Nov;59(11):3771-7. doi: 10.1128/aem.59.11.3771-3777.1993.
10
Microbial treatment of metal pollution--a working biotechnology?金属污染的微生物处理——一种可行的生物技术？
Trends Biotechnol. 1993 Aug;11(8):353-9. doi: 10.1016/0167-7799(93)90158-6.
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