Key Laboratory of Animal Biotechnology, Ministry of Agriculture, Northwest A&F University, Yangling 712100, China.
Int J Mol Sci. 2019 Apr 26;20(9):2071. doi: 10.3390/ijms20092071.
Retinoic acid (RA) plays a key role in pluripotent cell differentiation. In F9 embryonic carcinoma cells, RA can induce differentiation towards somatic lineages via the Ras-extracellular signal-regulated kinase (Ras/Erk) pathway, but the mechanism through which it induces the Erk1/2 phosphorylation is unclear. Here, we show that miR-485 is a positive regulator that targets α/β-hydrolase domain-containing protein 2 (Abhd2), which can result in Erk1/2 phosphorylation and triggers differentiation. RA up-regulates miR-485 and concurrently down-regulates Abhd2. We verified that Abhd2 is targeted by miR-485 and they both can influence the phosphorylation of Erk1/2. In summary, RA can mediate cell differentiation by phosphorylating Erk1/2 via miR-485 and Abhd2.
视黄酸（RA）在多能细胞分化中起着关键作用。在 F9 胚胎癌细胞中，RA 可以通过 Ras-细胞外信号调节激酶（Ras/Erk）途径诱导向体细胞谱系分化，但诱导 Erk1/2 磷酸化的机制尚不清楚。在这里，我们表明 miR-485 是一种正调节剂，可靶向α/β-水解酶结构域包含蛋白 2（Abhd2），导致 Erk1/2 磷酸化并触发分化。RA 上调 miR-485 并同时下调 Abhd2。我们验证了 Abhd2 是 miR-485 的靶标，它们都可以影响 Erk1/2 的磷酸化。总之，RA 可以通过 miR-485 和 Abhd2 磷酸化 Erk1/2 来介导细胞分化。
