Programa de Pós-Graduação Interdisciplinar em Ciências da Saúde, Universidade Federal de São Paulo, Santos 11015-020, Brazil.
Departamento de Biociências, Universidade Federal de São Paulo, Santos 11015-020, Brazil.
Molecules. 2019 Apr 27;24(9):1655. doi: 10.3390/molecules24091655.
Juçara berry is a potential inflammatory modulator, rich in dietary fiber, fatty acids, and anthocyanins. Considering this, we evaluated the high-fat diet (HFD) intake supplemented with different doses of freeze-dried juçara pulp on the TLR4 pathway. Twenty-seven male Wistar rats with ad libitum access to food and water were divided into four experimental groups: control standard chow group (C); high-fat diet control group (HFC); high-fat diet juçara 0.25% group (HFJ0.25%); and high-fat diet juçara 0.5% group (HFJ0.5%). The inflammatory parameters were analyzed by ELISA and Western blotting in liver and retroperitoneal adipose tissue (RET). The HFJ0.25% group had the energy intake, aspartate transaminase (AST) levels, and liver triacylglycerol accumulation reduced; also, the tumor necrosis factor α (TNF-α) and TNF receptor-associated factor 6 (TRAF6) expression in RET were reduced. However, there were no changes in other protein expressions in liver and adipose tissue. Adiposity and pNFκBp50 had a positive correlation in HFC and HFJ0.5%, but not in the C group and HFJ0.25%. The necrosis hepatic score did not change with treatment; however, the serum (AST) levels and the hepatic triacylglycerol were increased in HFC and HFJ0.5%. These results demonstrated that one week of HFD intake triggered pro-inflammatory mechanisms and liver injury. Additionally, 0.25% juçara prevented inflammatory pathway activation, body weight gain, and liver damage.
树莓果实是一种有潜力的炎症调节剂，富含膳食纤维、脂肪酸和花青素。考虑到这一点，我们评估了高脂肪饮食（HFD）摄入，并补充了不同剂量的冻干树莓果肉对 TLR4 途径的影响。27 只雄性 Wistar 大鼠自由进食和饮水，分为四个实验组：对照标准饲料组（C）；高脂肪饮食对照组（HFC）；高脂肪饮食树莓 0.25%组（HFJ0.25%）；和高脂肪饮食树莓 0.5%组（HFJ0.5%）。通过 ELISA 和 Western blot 在肝脏和腹膜后脂肪组织（RET）中分析炎症参数。HFJ0.25%组的能量摄入、天门冬氨酸转氨酶（AST）水平和肝脏三酰甘油积累减少；此外，RET 中的肿瘤坏死因子 α（TNF-α）和 TNF 受体相关因子 6（TRAF6）表达减少。然而，肝脏和脂肪组织中其他蛋白质的表达没有变化。肥胖与 HFC 和 HFJ0.5%中的 pNFκBp50 呈正相关，但在 C 组和 HFJ0.25%中则没有。肝坏死评分没有随治疗而变化；然而，HFC 和 HFJ0.5%中的血清（AST）水平和肝三酰甘油升高。这些结果表明，一周的 HFD 摄入引发了促炎机制和肝损伤。此外，0.25%的树莓可预防炎症途径激活、体重增加和肝损伤。
