Cannarella Rossella, Arato Iva, Condorelli Rosita A, Luca Giovanni, Barbagallo Federica, Alamo Angela, Bellucci Catia, Lilli Cinzia, La Vignera Sandro, Calafiore Riccardo, Mancuso Francesca, Calogero Aldo E
Department of Clinical and Experimental Medicine, University of Catania, 95123 Catania, Italy.
Department of Experimental Medicine, University of Perugia, 06123 Perugia, Italy.
J Clin Med. 2019 Apr 27;8(5):577. doi: 10.3390/jcm8050577.
Experimental evidence has shown that the IGF1 receptor (IGF1R) is involved in testicular development during embryogenesis. More recently, data gathered from mice granulosa cells and zebrafish spermatogonia suggest that IGF1R has a role in Follicle-stimulating hormone (FSH) signaling. No evidence has been reported on this matter in Sertoli cells (SCs) so far. The aim of the study was to evaluate the role, if any, of the IGF1R in FSH signaling in SCs. The effects of FSH exposure on myosin-phosphatase 1 (MYPT1), ERK 1/2, AKT, AKT, c-Jun N-terminal kinase (JNK) phosphorylation and on anti-Müllerian hormone (AMH), inhibin B and FSH receptor (FSHR) mRNA levels were assessed with and without the IGF1R inhibitor NVP-AEW541 in purified and functional porcine neonatal SCs. Pre-treatment with NVP-AEW541 inhibited the FSH-induced MYPT1 and ERK 1/2 phosphorylation, decreased the FSH-dependent Protein kinase B (AKT) phosphorylation, but did not affect the FSH-induced AKT and JNK phosphorylation rate. It also interfered with the FSH-induced AMH and FSHR down-regulation. No influence was observed on the FSH-stimulated Inhibin B gene expression. Conclusion. These findings support the role of theIGF1R in FSH signaling in porcine SCs. The possible influence of IGF1 stimulation on the FSH-mediated effects on SCs should be further explored.
实验证据表明，胰岛素样生长因子1受体（IGF1R）在胚胎发生过程中参与睾丸发育。最近，从小鼠颗粒细胞和斑马鱼精原细胞收集的数据表明，IGF1R在促卵泡激素（FSH）信号传导中发挥作用。迄今为止，在支持细胞（SCs）中尚未有关于此事的报道。本研究的目的是评估IGF1R在SCs的FSH信号传导中的作用（如果有）。在纯化且具有功能的猪新生SCs中，使用和不使用IGF1R抑制剂NVP - AEW541评估FSH暴露对肌球蛋白磷酸酶1（MYPT1）、ERK 1/2、AKT、AKT、c - Jun氨基末端激酶（JNK）磷酸化以及抗苗勒管激素（AMH）、抑制素B和FSH受体（FSHR）mRNA水平的影响。用NVP - AEW541预处理可抑制FSH诱导的MYPT1和ERK 1/2磷酸化，降低FSH依赖性蛋白激酶B（AKT）磷酸化，但不影响FSH诱导的AKT和JNK磷酸化率。它还干扰了FSH诱导的AMH和FSHR下调。未观察到对FSH刺激的抑制素B基因表达有影响。结论。这些发现支持IGF1R在猪SCs的FSH信号传导中的作用。应进一步探索IGF1刺激对SCs上FSH介导效应的可能影响。
