Wald Regina, Hess Claudia, Urbantke Verena, Wittek Thomas, Baumgartner Martina
Department of Farm Animal and Public Health in Veterinary Medicine, University Clinic for Ruminants, University of Veterinary Medicine, 1210 Wien, Austria.
Department of Farm Animal and Public Health in Veterinary Medicine, University Clinic for Poultry and Fish Medicine, University of Veterinary Medicine, 1210 Wien, Austria.
Animals (Basel). 2019 Apr 27;9(5):200. doi: 10.3390/ani9050200.
is considered as a major mastitis pathogen, with considerable epidemiological information on such infections while the epidemiology of coagulase-negative staphylococci (CNS) is more controversial. The aim of this study was to use matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) technology for identification of staphylococci isolated from bovine milk at species level and to characterize them in reference to presentation, somatic cell count (SCC), bacterial shedding (cfu) and antimicrobial resistance patterns. A total of 200 staphylococcal isolates ( = 100; CNS = 100) originating from aseptically collected quarter milk samples from different quarters of dairy cows were included in the study. They originated from cases of clinical (CM) and subclinical mastitis (SCM) or were isolated from milk with SCC ≤ 100,000 cells/mL in pure culture. We found staphylococci predominantly in cases of SCM ( = 120). In low-SCC cows, 12 and 32 CNS isolates were detected. Eighteen percent of each were associated with CM. Eleven CNS species were identified, ( = 26) and ( = 40) predominated. CNS, particularly those in low-SCC cows, showed higher MIC90 (minimal inhibitory concentration) values for penicillin, ampicillin, cefoperazone, pirlimycin and marbofloxacin. Based on the present results, a careful interpretation of laboratory results is recommended to avoid antimicrobial therapy of staphylococci without clinical relevance and to ensure prudent use of antimicrobials.
被认为是主要的乳腺炎病原体，关于此类感染有大量的流行病学信息，而凝固酶阴性葡萄球菌（CNS）的流行病学则更具争议性。本研究的目的是使用基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）技术在种水平上鉴定从牛乳中分离出的葡萄球菌，并根据临床表现、体细胞计数（SCC）、细菌脱落（cfu）和抗菌药物耐药模式对其进行特征描述。本研究共纳入了200株葡萄球菌分离株（金黄色葡萄球菌=100株；CNS=100株），这些分离株来自从奶牛不同乳区无菌采集的乳样。它们来源于临床乳腺炎（CM）和亚临床乳腺炎（SCM）病例，或从纯培养物中SCC≤100,000个细胞/mL的乳中分离得到。我们发现葡萄球菌主要存在于SCM病例中（n=120）。在低SCC奶牛中，检测到12株金黄色葡萄球菌和32株CNS分离株。每种分离株的18%与CM相关。鉴定出11种CNS菌种，其中表皮葡萄球菌（n=26）和腐生葡萄球菌（n=40）占主导。CNS，尤其是低SCC奶牛中的CNS，对青霉素、氨苄西林、头孢哌酮、吡利霉素和马波沙星显示出更高的MIC90（最低抑菌浓度）值。基于目前的结果，建议仔细解读实验室结果，以避免对无临床相关性的葡萄球菌进行抗菌治疗，并确保谨慎使用抗菌药物。
