可变亚组III的含碳水化合物免疫球蛋白λ轻链淀粉样纤维蛋白（AL）的结构研究
Structural studies of a carbohydrate-containing immunoglobulin-lambda-light-chain amyloid-fibril protein (AL) of variable subgroup III.
作者信息
Holm E, Sletten K, Husby G
出版信息
Biochem J. 1986 Nov 1;239(3):545-51. doi: 10.1042/bj2390545.
Abstract
The amino acid sequence of the variable region of a carbohydrate-containing amyloid-fibril protein MOL of immunoglobulin-light-chain type (AL) was elucidated. The sequence determination involved cleaving the protein with CNBr, BNPS-skatole, thermolysin and trypsin. The sequenced protein consisted of about 130 amino acid residues; however, gel-filtration and N-terminal analysis studies revealed AL proteins ranging in Mr from about 10,000 to 25,000. The oligosaccharide chain was found to be bound in the hypervariable region. By sequence homology to other lambda chains the AL protein MOL was shown to be of the V lambda III subgroup.
摘要
免疫球蛋白轻链型（AL）含碳水化合物的淀粉样纤维蛋白MOL可变区的氨基酸序列已被阐明。序列测定包括用溴化氰、BNPS-粪臭素、嗜热菌蛋白酶和胰蛋白酶切割该蛋白。测序的蛋白由约130个氨基酸残基组成；然而，凝胶过滤和N端分析研究显示，AL蛋白的相对分子质量在约10,000至25,000之间。发现寡糖链结合在高变区。通过与其他λ链的序列同源性分析，AL蛋白MOL被证明属于VλIII亚组。
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FEBS Lett. 1971 Dec 1;19(2):169-173. doi: 10.1016/0014-5793(71)80506-9.
2
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N Engl J Med. 1980 Jun 12;302(24):1333-43. doi: 10.1056/NEJM198006123022403.
3
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9
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10
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