Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI 48109-2125, USA.
Biomaterials. 2010 May;31(15):4304-12. doi: 10.1016/j.biomaterials.2010.01.145.
The goal of this study was to determine material effects on cartilage regeneration for scaffolds with the same controlled architecture. The 3D polycaprolactone (PCL), poly (glycerol sebacate) (PGS), and poly (1,8 octanediol-co-citrate) (POC) scaffolds of the same design were physically characterized and tissue regeneration in terms of cell phenotype, cellular proliferation and differentiation, and matrix production were compared to find which material would be most optimal for cartilage regeneration in vitro. POC provided the best support for cartilage regeneration in terms of tissue ingrowth, matrix production, and relative mRNA expressions for chondrocyte differentiation (Col2/Col1). PGS was seen as the least favorable material for cartilage based on its relatively high de-differentiation (Col1), hypertrophic mRNA expression (Col10) and high matrix degradation (MMP13, MMP3) results. PCL still provided microenvironments suitable for cells to be active yet it seemed to cause de-differentiation (Col1) of chondrocytes inside the scaffold while many cells migrated out, growing cartilage outside the scaffold.
本研究的目的是确定具有相同控制结构的支架的材料效应对软骨再生的影响。对具有相同设计的 3D 聚己内酯 (PCL)、聚 (癸二酸甘油酯) (PGS) 和聚 (1,8-辛二醇-柠檬酸酯) (POC) 支架进行物理特性表征，并从细胞表型、细胞增殖和分化以及基质产生等方面比较组织再生，以找出哪种材料最适合体外软骨再生。在组织内长入、基质产生以及软骨细胞分化（Col2/Col1）的相对 mRNA 表达方面，POC 为软骨再生提供了最佳支持。PGS 被认为是最不利于软骨的材料，因为它的去分化（Col1）、肥大 mRNA 表达（Col10）和高基质降解（MMP13、MMP3）结果。PCL 仍为细胞提供了适宜的微环境，但似乎会导致支架内软骨细胞的去分化（Col1），同时许多细胞迁移到支架外，在支架外生长软骨。
