Department of Molecular Genetics, Faculty of Science, Science and Research Branch, Islamic Azad University, Tehran, Iran.
Genetic Division, Department of Biology, Faculty of Sciences, University of Isfahan, Isfahan, Iran.
Mol Biol Rep. 2019 Jun;46(3):2679-2684. doi: 10.1007/s11033-019-04703-z. Epub 2019 Apr 30.
Survivin is one of the major members of Inhibitory Apoptotic Proteins (IAP) family. The functional anti-apoptotic and regulatory role of Survivin in the cell cycle had made it as an interesting candidate for tumor studies. Acute lymphoblastic leukemia is one of the most common malignancies of children that accounts for 30% of all the childhood malignancies. The purpose of this study was to investigate the prognostic importance of Survivin level in B-cell acute lymphoblastic leukemia patients of Iran in four different steps of disease in order to follow up its impact on various treatment ways, the development of the disease, and the response to treatment in the patients. The expression level of Survivin was evaluated in 85 patients with B-cell acute lymphoblastic leukemia and 85 healthy controls using Quantitative Real-Time Polymerase Chain Reaction. Also, western blot analysis was done to confirm the results. Based on our findings, the expression of Survivin showed a significant up-regulation in patients compared to controls. Therefore, a correlation between Survivin expression and the development pattern of B-cell acute lymphoblastic leukemia with a strong diagnostic efficiency (AUC-ROC, 0.8562) was observed. Therefore, it can be introduced as a potential marker for prognosis B-cell Acute Lymphoblastic Leukemia in the future.
Survivin 是凋亡抑制蛋白（IAP）家族的主要成员之一。Survivin 在细胞周期中具有功能性的抗凋亡和调节作用，使其成为肿瘤研究的一个有趣的候选者。急性淋巴细胞白血病是儿童中最常见的恶性肿瘤之一，占所有儿童恶性肿瘤的 30%。本研究的目的是在疾病的四个不同阶段，探讨 Survivin 水平在伊朗 B 细胞急性淋巴细胞白血病患者中的预后重要性，以观察其对各种治疗方法、疾病进展和患者治疗反应的影响。使用定量实时聚合酶链反应（qRT-PCR）评估 85 例 B 细胞急性淋巴细胞白血病患者和 85 例健康对照者 Survivin 的表达水平。还进行了 Western blot 分析以验证结果。根据我们的发现，与对照组相比，患者中 Survivin 的表达明显上调。因此，观察到 Survivin 表达与 B 细胞急性淋巴细胞白血病的发展模式之间存在相关性，具有较强的诊断效率（AUC-ROC，0.8562）。因此，它可以作为未来 B 细胞急性淋巴细胞白血病预后的潜在标志物。
