Amirmahani Farzane, Ebrahimi Nasim, Askandar Rafee Habib, Rasouli Eshkaftaki Marzieh, Fazeli Katayoun, Hamblin Michael R
Department of Molecular Biology and Microbiology, Faculty of Biological Sciences and Technology, University of Isfahan, Isfahan, Iran.
Research center, Sulaimani Polytechnic University, Sulaimani, Iraq.
Indian J Clin Biochem. 2024 Jul;39(3):322-330. doi: 10.1007/s12291-021-00999-6. Epub 2021 Aug 24.
Prostate cancer (PCa) is the second most common cancer in men throughout the world, and the main cause of cancer death. Long noncoding RNAs (lncRNAs) act as crucial regulators in many human cancers. In this research, we measured the expression level of novel lncRNAs and their associated micro-RNAs (miRNAs) in PCa. In the present research, three lncRNAs were selected using the Mitranscriptome projec (CAT2064, CAT2042, and CAT2164.2). Samples of prostate tissue (20 PCa, and 20 BPH) and blood (14 PCa, and 14 BPH) were collected and the Real-time Quantitative Polymerase Chain Reaction (RT-qPCR) was used to measure the expression levels of the lncRNAs and their associated miRNAs. Based on our results, CAT2064 was significantly increased and CAT2042 was significantly decreased in human PCa tissue in comparison with BPH tissue. To discriminate PCa from BPH, CAT2064 ( < 0.05; 0.8750 AUC-ROC) showed a better potential as a diagnostic molecular biomarker compared to CAT2042 ( < 0.05; 0.8454 AUC-ROC). Furthermore, RT-qPCR results measured in blood samples from PCa patients showed a higher expression level of CAT2064 ( < 0.0001; AUC-ROC value of 0.8914) in comparison to CAT2042. CAT2064 and CAT2042 showed a positive correlation with the expression of miR-5095 and miR-1273a (r = 0.02885, 0.3202; = 0.9413, 0.2266, respectively). CAT2064 and CAT2042 also had a negative correlation with miR-1304-3p and miR-1285-5p (r = - 0.3877, - 0.09330; = 0.15, 0.7311, respectively). Collectively, CAT2064 and CAT2042 and their miRNA targets may constitute a regulatory network in PCa, and could serve as novel biomarkers.
The online version contains supplementary material available at 10.1007/s12291-021-00999-6.
前列腺癌（PCa）是全球男性中第二常见的癌症，也是癌症死亡的主要原因。长链非编码RNA（lncRNAs）在许多人类癌症中起着关键调节作用。在本研究中，我们测量了PCa中新型lncRNAs及其相关微小RNA（miRNAs）的表达水平。在本研究中，使用Mitranscriptome项目选择了三种lncRNAs（CAT2064、CAT2042和CAT2164.2）。收集前列腺组织样本（20例PCa和20例良性前列腺增生症[BPH]）和血液样本（14例PCa和14例BPH），并使用实时定量聚合酶链反应（RT-qPCR）测量lncRNAs及其相关miRNAs的表达水平。根据我们的结果，与BPH组织相比，人类PCa组织中CAT2064显著升高，CAT2042显著降低。为了区分PCa和BPH，与CAT2042（P<0.05；曲线下面积[AUC-ROC]为0.8454）相比，CAT2064（P<0.05；AUC-ROC为0.8750）作为诊断分子生物标志物具有更好的潜力。此外，在PCa患者血液样本中测量的RT-qPCR结果显示，与CAT2042相比，CAT2064的表达水平更高（P<0.0001；AUC-ROC值为0.8914）。CAT2064和CAT2042与miR-5095和miR-1273a的表达呈正相关（r分别为0.02885、0.3202；P分别为0.9413、0.2266）。CAT2064和CAT2042与miR-1304-3p和miR-1285-5p也呈负相关（r分别为-0.3877、-0.09330；P分别为0.15、0.7311）。总体而言，CAT2064和CAT2042及其miRNA靶点可能在PCa中构成一个调控网络，并可作为新型生物标志物。
在线版本包含可在10.1007/s12291-021-00999-6获取的补充材料。
