Department of Internal Medicine, Seoul National University College of Medicine, 101 Daehak-ro, Jongno-gu, Seoul, 03080, Republic of Korea.
Department of Internal Medicine, Seoul National University Hospital, Seoul, Republic of Korea.
Obes Surg. 2019 Aug;29(8):2399-2408. doi: 10.1007/s11695-019-03858-9.
Ileal transposition (IT) is an experimental surgery to investigate the role of the distal ileum in Roux-en-Y gastric bypass (RYGB) surgery. To systematically investigate the dynamic adaptation process of the ileum after IT, we performed transcriptome analyses of the transposed ileum compared with the ileum in situ at different postoperative time points.
Sprague-Dawley rats fed a chow diet underwent IT or sham surgery. One and 4 weeks after IT or sham surgery, total RNA was extracted from the ileal tissue and subjected to transcriptome analyses using microarray.
Principal component analysis showed that the difference between weeks 1 and 4 was the largest, and the differences between the IT and sham groups were larger in week 4 than in week 1. We identified 1792 differentially expressed genes (DEGs) between IT and sham ileal tissues, including 659 and 1133 DEGs in weeks 1 and 4, respectively. Interestingly, only 45 and 24 DEGs were commonly up- or downregulated in weeks 1 and 4, indicating a marked transition during the adaptation process. Functional enrichment and network analyses showed that structural adaptation predominantly occurred in week 1, while metabolic and immune adaptations predominantly occurred in week 4. These analyses further revealed potential components that modulate structural adaptation (e.g., extracellular matrix) in week 1 and metabolic (e.g., glucose transporter) and immune (e.g., Th17 cells) adaptations in week 4.
The transposed distal ileum underwent dynamic adaptation processes that may help explain the metabolic changes after RYGB.
回肠转位（IT）是一种实验性手术，旨在研究远端回肠在 Roux-en-Y 胃旁路（RYGB）手术中的作用。为了系统地研究 IT 后回肠的动态适应过程，我们对转位回肠和原位回肠在不同术后时间点进行了转录组分析。
给予标准饮食的 Sprague-Dawley 大鼠接受 IT 或假手术。在 IT 或假手术后 1 周和 4 周，从回肠组织中提取总 RNA，并使用微阵列进行转录组分析。
主成分分析显示，第 1 周和第 4 周之间的差异最大，IT 组和假手术组之间的差异在第 4 周大于第 1 周。我们鉴定了 IT 回肠组织和假手术回肠组织之间的 1792 个差异表达基因（DEGs），包括第 1 周和第 4 周分别有 659 个和 1133 个 DEGs。有趣的是，只有 45 个和 24 个 DEGs在第 1 周和第 4 周分别呈上调或下调表达，表明在适应过程中存在明显的转变。功能富集和网络分析显示，结构适应主要发生在第 1 周，而代谢和免疫适应主要发生在第 4 周。这些分析进一步揭示了调节第 1 周结构适应的潜在成分（例如，细胞外基质）和第 4 周代谢（例如，葡萄糖转运蛋白）和免疫（例如，Th17 细胞）适应的潜在成分。
转位的远端回肠经历了动态适应过程，这可能有助于解释 RYGB 后的代谢变化。
