Department of Internal Medicine, Seoul National University College of Medicine, 101 Daehak-ro, Jongno-gu, Seoul, 03080, Republic of Korea.
Department of Internal Medicine, Seoul National University Bundang Hospital, Seongnam, Republic of Korea.
Sci Rep. 2021 Jan 27;11(1):2310. doi: 10.1038/s41598-021-81866-5.
Vertical sleeve gastrectomy (VSG) is the most commonly performed bariatric/metabolic surgery, exhibiting a high rate of diabetes remission in humans. To elucidate the molecular mechanisms of VSG, we performed transcriptomic analysis of the liver, fat, and muscle in VSG mice. C57BL/6 mice fed a high-fat diet were randomly assigned to sham or VSG surgery. The sham-operated mice were fed ad libitum (sham group) or pair-fed (sham-PF group) matching their food intake to the VSG-operated mice. Comparative transcriptomic analysis of the liver, fat, and muscle using RNA sequencing was performed. VSG reduced body weight and improved glucose tolerance compared to the sham group, but not more than the sham-PF group. Improvement in fatty liver and adipose tissue inflammation was comparable between VSG and sham-PF. However, global gene expression profiles showed distinctive changes in the liver, fat, and muscle of the VSG group compared to both the sham or sham-PF groups. The liver showed the most prominent gene expression changes. Immune response-related pathways were commonly upregulated in the three organs of the VSG group compared to the sham or sham-PF. VSG induces organ-specific gene expression changes in the liver, fat, and muscle, which may play critical roles in metabolic improvements after VSG.
袖状胃切除术（VSG）是最常进行的减肥/代谢手术，在人类中显示出很高的糖尿病缓解率。为了阐明 VSG 的分子机制，我们对 VSG 小鼠的肝脏、脂肪和肌肉进行了转录组分析。将高脂肪饮食喂养的 C57BL/6 小鼠随机分配至假手术或 VSG 手术组。假手术组给予自由进食（假手术组）或按与 VSG 手术组相同的进食量进行配对进食（假手术-PF 组）。使用 RNA 测序对肝脏、脂肪和肌肉进行了转录组比较分析。与假手术组相比，VSG 降低了体重并改善了葡萄糖耐量，但不如假手术-PF 组。VSG 与假手术-PF 组在改善脂肪肝和脂肪组织炎症方面相当。然而，与假手术或假手术-PF 组相比，VSG 组的肝脏、脂肪和肌肉的整体基因表达谱显示出明显的变化。肝脏显示出最显著的基因表达变化。与假手术或假手术-PF 组相比，VSG 组三个器官中的免疫反应相关途径普遍上调。VSG 诱导肝脏、脂肪和肌肉中特定于器官的基因表达变化，这可能在 VSG 后代谢改善中起关键作用。
