Masaryk University, Faculty of Medicine, Clinic of Stomatology, Brno, Czech Republic.
Masaryk University, Institute of Biostatistics and Analyses, Brno, Czech Republic.
Braz Oral Res. 2019 Apr 25;33:e034. doi: 10.1590/1807-3107bor-2019.vol33.0034.
Specific variants in genes that encode adipokines and their mRNA and protein expression were previously studied in type 2 diabetes mellitus (T2DM) and obesity, and similar studies have been performed for chronic periodontitis (CP). The aim of this case-control study was to investigate the possible impacts of adiponectin (ADIPOQ), leptin (LEP) and its receptor (LEPR), and resistin (RETN) on the etiopathogenesis of CP. Examinations were performed on 118 non-periodontitis healthy subjects (healthy controls, HC), 205 healthy individuals with CP (H + CP) and 86 type 2 diabetes patients with CP (T2DM + CP). Variants within the ADIPOQ (rs2241766, rs1501299), LEP (rs13228377, rs2167270), LEP receptor (rs1805096), and RETN (rs1862513) genes were determined by qPCR. In addition, the plasma levels of ADIPOQ, LEP, and RETN were analysed by ELISA for 80 individuals. The genotype frequencies of the SNP ADIPOQ +45G/T (rs2241766) differed between the HC and H + CP groups (p=0.03, pcorr>0.05), and carriers of the TT genotype had a lower risk of developing CP compared to carriers of the GG or TG genotypes (p<0.01, pcorr>0.05). However, there were no significant differences in the plasma levels of ADIPOQ, LEP or RETN between the study groups (p > 0.05). Plasma levels of the adipokines were also independent of the gene profiles (p > 0.05). Adipokine plasma levels did not change in patients with H + CP/T2DM + CP compared to HC, but we did identify a specific polymorphism in the ADIPOQ gene that was associated with CP. Although the ADIPOQ +45G/T (rs2241766) gene variant may be a candidate biomarker for CP, further research is required in larger populations with different ethnic backgrounds before any final conclusions can be drawn about the role of this gene in CP.
先前已经在 2 型糖尿病（T2DM）和肥胖症中研究了编码脂肪因子及其 mRNA 和蛋白质表达的基因中的特定变体，并且已经对慢性牙周炎（CP）进行了类似的研究。本病例对照研究的目的是探讨脂联素（ADIPOQ）、瘦素（LEP）及其受体（LEPR）和抵抗素（RETN）对 CP 发病机制的可能影响。对 118 名非牙周炎健康受试者（健康对照组，HC）、205 名健康 CP 患者（H + CP）和 86 名 2 型糖尿病 CP 患者（T2DM + CP）进行了检查。通过 qPCR 确定了 ADIPOQ（rs2241766、rs1501299）、LEP（rs13228377、rs2167270）、LEP 受体（rs1805096）和 RETN（rs1862513）基因内的变体。此外，对 80 名个体的 ADIPOQ、LEP 和 RETN 血浆水平进行了 ELISA 分析。SNP ADIPOQ +45G/T（rs2241766）的基因型频率在 HC 和 H + CP 组之间存在差异（p=0.03，pcorr>0.05），与 GG 或 TG 基因型相比，TT 基因型的个体发生 CP 的风险较低（p<0.01，pcorr>0.05）。然而，研究组之间 ADIPOQ、LEP 或 RETN 的血浆水平没有差异（p>0.05）。脂肪因子的血浆水平也与基因谱无关（p>0.05）。与 HC 相比，H + CP/T2DM + CP 患者的脂肪因子血浆水平没有变化，但我们确实在 ADIPOQ 基因中发现了一个与 CP 相关的特定多态性。虽然 ADIPOQ +45G/T（rs2241766）基因变体可能是 CP 的候选生物标志物，但在得出关于该基因在 CP 中的作用的任何最终结论之前，需要在具有不同种族背景的更大人群中进行进一步研究。
