Clinical Pathology and Haematology Department, Faculty of Medicine, Ain Shams University Biomedical Research Department, Cairo, Egypt.
Neurosurgery Department, Faculty of Medicine, Mansoura University, Mansoura, Egypt.
J Cell Biochem. 2019 Sep;120(9):15288-15296. doi: 10.1002/jcb.28796. Epub 2019 Apr 30.
To investigate the correlation of homeobox (HOX) transcript antisense RNA expression with clinicopathological features and the clinical prognosis of the patients with chromosome 12p abnormalities associated acute myeloid leukemia (AML). We also investigate the association of 12p chromosomal on the expression of HOTAIR, miRNA-193a, and c-kit gene as targeting genes for HOTAIR in AML.
AML patients with 12p chromosomal abnormalities were recruited and compared to AML with other chromosomal abnormalities rather than 12p. The long noncoding RNA (lncRNA) "HOTAIR," miR-193a, and c-Kit genes expression were measured in bone marrow samples using Syber green based real-time polymerase chain reaction.
We found a significant difference for the expression levels of HOTAIR, c-kit, and miR-193a between 12p abnormalities associated AML and those without. The survival analysis revealed that patient's with low expression levels of HOTAIR and c-kit had significantly better survival and leukemia free survival. In contrast, miR-193a was associated with better overall survival but not leukemia free survival.
12p abnormalities associated AML were associated with worse prognosis. Our results proved that HOTAIR, miR-193a, and c-kit genes are independent prognostic predictors in 12p chromosomal associated AML; therefore it may represent a novel therapeutic application in AML in the future.
研究同源盒（HOX）转录反义 RNA 表达与伴有 12 号染色体 p 区异常的急性髓系白血病（AML）患者临床病理特征和临床预后的相关性。我们还研究了 12p 染色体上 HOX 转录反义 RNA 的表达与 HOTAIR、miR-193a 和 c-kit 基因的相关性，这些基因是 AML 中 HOX 转录反义 RNA 的靶向基因。
招募伴有 12p 染色体异常的 AML 患者，并与伴有其他染色体异常而不是 12p 异常的 AML 患者进行比较。采用 Syber 绿色实时聚合酶链反应检测骨髓样本中长链非编码 RNA（lncRNA）“HOTAIR”、miR-193a 和 c-Kit 基因的表达。
我们发现伴有 12p 异常的 AML 患者与不伴有 12p 异常的 AML 患者之间 HOTAIR、c-kit 和 miR-193a 的表达水平存在显著差异。生存分析显示，HOTAIR 和 c-kit 低表达的患者生存和无白血病生存明显更好。相比之下，miR-193a 与总生存相关，但与无白血病生存无关。
伴有 12p 异常的 AML 与预后不良相关。我们的研究结果证明，HOTAIR、miR-193a 和 c-kit 基因是 12p 染色体相关 AML 的独立预后预测因子；因此，它可能代表未来 AML 的一种新的治疗应用。
