a Department of Food Science and Nutrition , Zhejiang University , Hangzhou , People's Republic of China.
b Zhejiang Key Laboratory for Agro-Food Processing , Zhejiang University , Hangzhou , People's Republic of China.
Free Radic Res. 2019 May;53(5):562-573. doi: 10.1080/10715762.2019.1613534. Epub 2019 May 16.
The main flavonoid components of Radix Tetrastigma (RTF) were extracted and identified by UPLC-TOF/MS. , RTF prevented inflammation in RAW 264.7 cells by suppressing morphological (both cell and nucleus) changes, and decreasing nitric oxide (NO), inducible nitric oxide synthase (iNOS), and cyclooxygenase-2 (COX-2) contents. Exposure to LPS also leads to oxidant damage, and RTF alleviated damage to mitochondria, decreased O accumulation, and restored the glutathione level. RTF intervention decreased the expression of c-Jun N-terminal kinase (JNK) and p38 phosphorylation, accompanied by downregulation of nuclear factor erythroid 2-related factor 2 (Nrf2) and forkhead box protein O1 (FoxO1). , aging of by paraquat (PQ) was observed through lifespan, lipofuscin, and enzyme analysis. RTF protected against damage in N2 worms but not in daf-16 mutants. Gene expression was further assessed, and p38/PMK-1 and Nrf2/SKN-1 expression in worms was suppressed by PQ, which was reversed by RTF treatment. Together, these results suggested that RTF could help ameliorate inflammation-induced damage through JNK, p38 and Nrf2 pathways.
通过 UPLC-TOF/MS 提取和鉴定了落新妇苷（RTF）的主要黄酮类成分。RTF 通过抑制 RAW 264.7 细胞的形态变化（细胞和细胞核），降低一氧化氮（NO）、诱导型一氧化氮合酶（iNOS）和环氧化酶-2（COX-2）含量来预防炎症。暴露于 LPS 还会导致氧化损伤，而 RTF 减轻了线粒体损伤，减少了 O 的积累，并恢复了谷胱甘肽水平。RTF 干预降低了 c-Jun N-末端激酶（JNK）和 p38 的磷酸化，同时下调了核因子红细胞 2 相关因子 2（Nrf2）和叉头框蛋白 O1（FoxO1）。通过寿命、脂褐素和酶分析观察到对百草枯（PQ）引起的衰老的影响。RTF 保护 N2 蠕虫免受损伤，但对 daf-16 突变体无效。进一步评估了基因表达，PQ 抑制了蠕虫中 p38/PMK-1 和 Nrf2/SKN-1 的表达，而 RTF 处理则逆转了这一表达。综上所述，这些结果表明 RTF 可以通过 JNK、p38 和 Nrf2 通路帮助改善炎症引起的损伤。
