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D-半胱氨酸对变形链球菌和血链球菌双物种生物膜的影响。

Effect of D-cysteine on dual-species biofilms of Streptococcus mutans and Streptococcus sanguinis.

机构信息

State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, 610041, China.

Department of Cariology and Endodontics, West China Hospital of Stomatology, Sichuan University, Chengdu, 610041, China.

出版信息

Sci Rep. 2019 Apr 30;9(1):6689. doi: 10.1038/s41598-019-43081-1.

DOI:10.1038/s41598-019-43081-1
PMID:31040318
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6491432/
Abstract

Dental caries is a highly prevalent disease worldwide. It is caused by the cariogenic biofilms composed of multiple dynamic bacteria on dental surface. Streptococcus mutans and Streptococcus sanguinis are resident members within the biofilms and an antagonistic relationship has been shown between these two species. S. mutans, as the major causative microorganism of dental caries, has been reported to be inhibited by free D-cysteine (D-Cys). However, whether D-Cys could affect S. sanguinis and the interspecies relationship between S. mutans and S. sanguinis remains unknown. The aim of the current study was to investigate the effect of D-Cys on the growth and cariogenicity of dual-species biofilms formed by S. mutans and S. sanguinis. We measured dual-species biofilms biomass, metabolic activity, lactate production. We also detected the biofilms structure, the ratio of live/dead bacteria, extracellular polysaccharide (EPS) synthesis and bacterial composition in the dual-species biofilms. We found that D-Cys could reduce the metabolic activity and lactic acid production of dual-species biofilms (p < 0.05). In addition, biofilms formation, the proportion of S. mutans in dual-species biofilms, and EPS synthesis were decreased with D-Cys treatment. The results suggested that D-Cys could inhibit the growth and cariogenic virulence of dual-species biofilms formed by S. mutans and S. sanguinis, indicating the potential of D-Cys in clinical application for caries prevention and treatment.

摘要

龋齿是一种在全球范围内广泛流行的疾病。它是由牙齿表面多种动态细菌组成的致龋生物膜引起的。变形链球菌和血链球菌是生物膜中的常驻成员，这两种细菌之间存在拮抗关系。变形链球菌作为龋齿的主要致病微生物，已被报道被游离 D-半胱氨酸（D-Cys）抑制。然而，D-Cys 是否会影响血链球菌以及变形链球菌和血链球菌之间的种间关系尚不清楚。本研究旨在探讨 D-Cys 对变形链球菌和血链球菌形成的双物种生物膜生长和致龋性的影响。我们测量了双物种生物膜的生物量、代谢活性、乳酸生成。我们还检测了生物膜结构、活/死细菌比例、细胞外多糖（EPS）合成和双物种生物膜中的细菌组成。结果发现，D-Cys 可降低双物种生物膜的代谢活性和乳酸生成（p<0.05）。此外，D-Cys 处理可减少生物膜形成、双物种生物膜中变形链球菌的比例和 EPS 合成。结果表明，D-Cys 可抑制变形链球菌和血链球菌形成的双物种生物膜的生长和致龋毒力，表明 D-Cys 在龋齿预防和治疗的临床应用中的潜力。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c495/6491432/89a140376f72/41598_2019_43081_Fig7_HTML.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c495/6491432/8adbfc3c25b6/41598_2019_43081_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c495/6491432/4d0ad3ef2903/41598_2019_43081_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c495/6491432/89a140376f72/41598_2019_43081_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c495/6491432/9dd60893084b/41598_2019_43081_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c495/6491432/3ccdb293e952/41598_2019_43081_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c495/6491432/4e27ddc88c55/41598_2019_43081_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c495/6491432/d6a4c7afe745/41598_2019_43081_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c495/6491432/8adbfc3c25b6/41598_2019_43081_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c495/6491432/4d0ad3ef2903/41598_2019_43081_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c495/6491432/89a140376f72/41598_2019_43081_Fig7_HTML.jpg

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