Häusler R E, Holtum J A, Latzko E
Eur J Biochem. 1987 Mar 16;163(3):619-26. doi: 10.1111/j.1432-1033.1987.tb10911.x.
NADP malic enzyme (EC 1.1.1.40) was extracted and partially purified from the green leaves of Zea mays var. Felix and from wheat germ. The active inorganic carbon species for both enzymes was, in contrast to an earlier report, CO2 not HCO3-. The apparent Km, CO2 for the maize enzyme was 1.2 mM and the apparent Km, CO2 for the wheat germ preparation was 4.2 mM under conditions of substrate saturation, pH 7.3 and 17 degrees C. These observations support the views that CO2, rather than HCO3- as has been suggested, is produced in bundle-sheath chloroplasts and that the reaction mechanism catalysed by plant cytosolic and chloroplastic NADP malic enzymes is similar to that proposed for avian NADP malic enzymes.
从费利克斯玉米（Zea mays var. Felix）的绿叶和小麦胚芽中提取并部分纯化了NADP苹果酸酶（EC 1.1.1.40）。与早期报告相反，这两种酶的活性无机碳物种是CO₂而非HCO₃⁻。在底物饱和、pH 7.3和17℃的条件下，玉米酶的表观Km（CO₂）为1.2 mM，小麦胚芽制剂的表观Km（CO₂）为4.2 mM。这些观察结果支持以下观点：与之前所认为的相反，CO₂是在维管束鞘叶绿体中产生的，而非HCO₃⁻；并且植物胞质和叶绿体NADP苹果酸酶催化的反应机制与鸟类NADP苹果酸酶所提出的机制相似。
