Chemical Engineering, School for Engineering of Matter, Transport and Energy, Arizona State University, Tempe, AZ, USA.
Biodesign Center for Applied Structural Discovery, Biodesign Institute, Arizona State University, Tempe, AZ, USA.
Methods Mol Biol. 2021;2215:287-297. doi: 10.1007/978-1-0716-0966-8_13.
Microcrystal Electron Diffraction (MicroED) enables structure determination of very small crystals that are much too small to be of use for other conventional diffraction techniques. MicroED has been used to determine the structures of many proteins and small organic molecules, and the technique can be performed on most standard cryo-TEM instruments equipped with high-speed detectors capable of collecting electron diffraction data. Here, we present protocols for MicroED sample preparation and data collection for protein microcrystals.
微晶体电子衍射(MicroED)能够确定非常小的晶体的结构,这些晶体小到无法使用其他常规衍射技术。MicroED 已被用于确定许多蛋白质和小分子的结构,并且该技术可以在配备有高速探测器的大多数标准 cryo-TEM 仪器上进行,这些探测器能够收集电子衍射数据。在这里,我们提供了用于蛋白质微晶体的 MicroED 样品制备和数据收集的方案。