从阿尔及利亚选定的乳制品中分离出来的：患病率及对抗生素的敏感性。
isolated from selected dairies of Algeria: Prevalence and susceptibility to antibiotics.
作者信息
Matallah Asmaa Manel, Bouayad Leila, Boudjellaba Sofiane, Mebkhout Faiza, Hamdi Taha Mossadak, Ramdani-Bouguessa Nadjia
机构信息
Laboratory of Food Hygiene and Quality Insurance System (HASAQ), Higher National Veterinary School, Rue Issad Abbes, Oued Smar, Algiers 16000, Algeria.
Research Laboratory Management of Local Animal Resources (GRAAL), Higher National Veterinary School, Rue Issad Abbes, Oued Smar, Algiers 16000, Algeria.
出版信息
Vet World. 2019;12(2):205-210. doi: 10.14202/vetworld.2019.205-210. Epub 2019 Feb 8.
AIM
The objectives of this study were to assess the prevalence of in raw milk in Algerian dairies, to study the effect of seasons on the contamination of milk and the susceptibility of isolated strains to antibiotics, and to estimate the risk on the health consumer.
MATERIALS AND METHODS
The ISO method 6888-1 (1) was used for screening. Antimicrobial susceptibility to the 11most used antibiotics in veterinary medicine was assessed using the disk diffusion assay.
RESULTS
The overall prevalence was 31.56% (95/301); 34.84% (85/244) from raw milk collectors cisterns (MCC), 22.73% (5/22) from mixing tank milk before pasteurization, and 14.29% (5/35) from pasteurized tank milk (p<0.05). A significant difference (p<0.001) of contamination on MCC was observed between dairies without season influence (p≥0.05). It was observed that 49.47% of isolates were resistant to penicillin, 5.26% to tetracycline, 4.21% to erythromycin, 3.15% to neomycin, 2.10% to cefoxitin, 2.10% to clindamycin, and 1.05% to ofloxacin. No resistance was observed for vancomycin, gentamicin, chloramphenicol, and trimethoprim-sulfamethoxazole.
CONCLUSION
A high prevalence of from MCC was observed without significant effect of season. The pasteurization does not ensure the elimination of bacteria in all samples. Half of the isolates were resistant to penicillin. These findings emphasize the importance of control in Algerian milk industry at different levels to improve public health.
目的
本研究的目的是评估阿尔及利亚乳制品厂原料乳中[具体细菌名称未给出]的流行情况，研究季节对牛奶污染及分离菌株对抗生素敏感性的影响，并估计对消费者健康的风险。
材料与方法
采用ISO 6888-1(1)方法进行[具体细菌名称未给出]筛查。使用纸片扩散法评估对兽医学中11种最常用抗生素的抗菌敏感性。
结果
总体流行率为31.56%（95/301）；原料乳收集罐（MCC）中为34.84%（85/244），巴氏杀菌前混合罐牛奶中为22.73%（5/22），巴氏杀菌罐牛奶中为14.29%（5/35）（p<0.05）。在不受季节影响的乳制品厂之间，MCC上的污染存在显著差异（p<0.001）（p≥0.05）。观察到49.47%的[具体细菌名称未给出]分离株对青霉素耐药，5.26%对四环素耐药，4.21%对红霉素耐药，3.15%对新霉素耐药，2.10%对头孢西丁耐药，2.10%对克林霉素耐药，1.05%对氧氟沙星耐药。未观察到对万古霉素、庆大霉素、氯霉素和甲氧苄啶-磺胺甲恶唑的耐药性。
结论
观察到MCC中[具体细菌名称未给出]的流行率很高，且季节影响不显著。巴氏杀菌不能确保消除所有样品中的细菌。一半的分离株对青霉素耐药。这些发现强调了在阿尔及利亚牛奶行业不同层面进行[具体细菌名称未给出]控制以改善公众健康的重要性。
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