Kojima N, Matsukura N, Shimizu Y, Yoshiyuki T, Nishi K, Wada M, Tokunaga A, Tanaka N, Onda M, Asano G
Jpn J Cancer Res. 1987 Feb;78(2):126-33.
Intestinal metaplasia (IM) in the glandular stomach of male Wistar rats induced by oral administration of N-ethyl-N'-nitro-N-nitrosoguanidine (ENNG) and/or intubation of 0.1N sodium hydroxide (NaOH) was studied as follows. Experiment I, sequential study: Rats in group I were given 100 micrograms/ml ENNG in drinking water for 12 weeks. Rats in group II were given 5 ml of 0.1N NaOH by gastric intubation once a week for 12 weeks. Group III control rats were given tap water. Rats were killed from week 1 until week 69 sequentially. IM was first found at week 26 in group I and at week 58 in groups II and III, its incidence being significantly higher in group I than in the other two groups (P less than 0.01), but without any difference between group II and group III. Experiment II, two-stage carcinogenesis: Rats in groups I and II were treated in the same way as in experiment I, while rats in group III were given 100 micrograms/ml ENNG for 12 weeks, followed by 0.1N NaOH once a week for 12 weeks intragastrically. All rats were killed at week 56. The numbers of metaplastic glands in groups I and III were higher than in group II. Gastric carcinomas were induced in all groups of rats treated with ENNG. The results of these two experiments show that IM is effectively induced by a carcinogen but is not enhanced by regeneration induced by alkaline treatment.
研究了通过口服N-乙基-N'-硝基-N-亚硝基胍（ENNG）和/或经胃管注入0.1N氢氧化钠（NaOH）诱导雄性Wistar大鼠腺胃肠化生（IM）的情况，具体如下。实验一，序贯研究：第一组大鼠饮用含100微克/毫升ENNG的水12周。第二组大鼠每周经胃管注入5毫升0.1N NaOH，共12周。第三组对照大鼠饮用自来水。从第1周开始依次处死大鼠，直至第69周。第一组在第26周首次发现肠化生，第二组和第三组在第58周发现，第一组的发生率显著高于其他两组（P<0.01），但第二组和第三组之间无差异。实验二，两阶段致癌作用：第一组和第二组大鼠的处理方式与实验一相同，而第三组大鼠饮用含100微克/毫升ENNG的水12周，随后每周经胃内注入0.1N NaOH，共12周。所有大鼠在第56周处死。第一组和第三组的化生腺数量高于第二组。用ENNG处理的所有大鼠组均诱发了胃癌。这两个实验的结果表明，致癌物可有效诱导肠化生，但碱性处理诱导的再生不会增强肠化生。
