Cary Daniele C, Rheinberger Mona, Rojc Ajda, Peterlin B Matija
Department of Medicine, University of California at San Francisco, San Francisco, California.
AIDS Res Hum Retroviruses. 2019 Aug;35(8):710-717. doi: 10.1089/AID.2019.0039. Epub 2019 May 29.
While the roles in HIV transcription of many cyclin-dependent kinases (CDKs) have been well defined, little is known about the impact of mediator kinases (MDKs), CDK8 and CDK19, in this process. Mediator complexes containing CDK8 or CDK19 repress or activate the expression of selected genes. The aim of this study was to investigate the role of MDKs in HIV transcription. siRNA knockdown of both MDKs had no effect on HIV transcription. This result was confirmed using two MDK inhibitors, Cortistatin A (CA) and Senexin A (SnxA). Furthermore, neither CA nor SnxA inhibited viral reactivation in Jurkat cell models of HIV latency. Taken together, these results indicate that MDKs are not required for HIV transcription.
虽然许多细胞周期蛋白依赖性激酶（CDK）在HIV转录中的作用已得到明确界定，但关于中介激酶（MDK）、CDK8和CDK19在此过程中的影响却知之甚少。含有CDK8或CDK19的中介复合物可抑制或激活特定基因的表达。本研究的目的是探讨MDK在HIV转录中的作用。对两种MDK进行小干扰RNA（siRNA）敲低对HIV转录没有影响。使用两种MDK抑制剂可待因A（CA）和塞内辛A（SnxA）证实了这一结果。此外，在HIV潜伏的Jurkat细胞模型中，CA和SnxA均未抑制病毒重新激活。综上所述，这些结果表明HIV转录不需要MDK。
