Gansu Agricultural University, College of Veterinary Medicine, Lanzhou, 730070, China.
Anning Branch Lanzhou Genegal Hospital, Lanzhou, 730070, China.
Virol J. 2019 May 2;16(1):56. doi: 10.1186/s12985-019-1167-z.
Tetraparvovirus, formerly known as Partetravirus, is a newly discovered genus in the family Parvoviridae that is considered phylogenetically distinct from other parvoviruses. However, nothing is known about the prevalence of Tetraparvovirus in special livestock living on the Qinghai-Tibet Plateau of China, such as Tibetan pigs and Tibetan sheep. A pair of special primers was designed based on the conserved regions in the genome of ungulate tetraparvovirus 2 (P-PARV4) and ungulate tetraparvovirus 4 (O-PARV4) and was used to detect P-PARV4 in domestic pigs and Tibetan pigs and O-PARV4 in ovines and Tibetan sheep. The results showed a 15.59 and 9.38% prevalence of P-PARV4 in domestic pigs (18.96% in Gansu Province and 11.76% in Qinghai Province) and Tibetan pigs (14.28% in Gansu Province and 4.44% in Qinghai Province), respectively, and a 7.31 and 5.20% prevalence of O-PARV4 in ovines (6.61% in Gansu Province and 8.00% in Qinghai Province) and Tibetan sheep (4.55% in Gansu Province and 5.50% in Qinghai Province), respectively. The prevalence of P-PARV4 was 14.76% (31/210) for ≤1-month-old pigs and 10.58% (20/189) for > 1-month-old pigs, and the positive rates of O-PARV4 were 7.65% (18/235) for ≤1-month-old sheep and 5.05% (11/218) for > 1-month-old sheep. The phylogenetic analysis of NS1, VP1, VP2 and the whole PARV4-related provirus genome demonstrated that both P-PARV4 and O-PARV4 sequences in this study were more closely related to the sequences of other strains discovered in the same genus of animals. The identity analyses for the full-length VP2 genomes of O-PARV4 revealed 98.84-100.00% sequence identity among the 7 strains and the previously reported strain, which was 98.60-99.28% for P-PARV4. In the present study, for the first time, we have provided comprehensive information regarding the widespread infection of P-PARV4 and O-PARV4 in special livestock on the Qinghai-Tibet Plateau in China. Our present findings highlight the importance of epidemiologic surveillance to limit the spread of Tetraparvovirus in livestock at high altitudes in China.
细小病毒，以前称为 Partetravirus，是细小病毒科中一个新发现的属，在系统发生上被认为与其他细小病毒明显不同。然而，对于生活在中国青藏高原的特种家畜（如藏猪和藏羊）中细小病毒的流行情况还知之甚少。根据偶蹄动物细小病毒 2（P-PARV4）和偶蹄动物细小病毒 4（O-PARV4）基因组中的保守区设计了一对特殊引物，用于检测国内猪和藏猪中的 P-PARV4 和绵羊和藏羊中的 O-PARV4。结果显示，国内猪的 P-PARV4 流行率为 15.59%（甘肃省 18.96%，青海省 11.76%）和藏猪的 9.38%（甘肃省 14.28%，青海省 4.44%），绵羊的 O-PARV4 流行率为 7.31%（甘肃省 6.61%，青海省 8.00%）和藏羊的 5.20%（甘肃省 4.55%，青海省 5.50%）。P-PARV4 的阳性率为≤1 月龄猪的 14.76%（31/210）和>1 月龄猪的 10.58%（20/189），O-PARV4 的阳性率为≤1 月龄绵羊的 7.65%（18/235）和>1 月龄绵羊的 5.05%（11/218）。NS1、VP1、VP2 和整个 PARV4 相关前病毒基因组的系统发育分析表明，本研究中 P-PARV4 和 O-PARV4 序列与同一属动物中其他菌株的序列更为密切相关。O-PARV4 全长 VP2 基因组的同一性分析显示，7 株与之前报道的菌株之间的序列同一性为 98.84-100.00%，而 P-PARV4 为 98.60-99.28%。在本研究中，我们首次提供了关于中国青藏高原特种家畜中 P-PARV4 和 O-PARV4 广泛感染的综合信息。我们的研究结果强调了进行流行病学监测以限制中国高原地区家畜中细小病毒传播的重要性。
