Engineering Research Center of Molecular and Neuro Imaging of the Ministry of Education, School of Life Science and Technology, Xidian University, Xi'an, Shaanxi, PR China.
Shaanxi Key Laboratory of Ischemic Cardiovascular Disease, Shaanxi Key Laboratory of Brain Disorders, Institute of Basic and Translational Medicine, Xi'an Medical University, Xi' an, Shaanxi, PR China.
Nanomedicine. 2019 Jul;19:136-144. doi: 10.1016/j.nano.2019.04.005. Epub 2019 Apr 29.
Fluorescent probes conjugated to peptide or antibody directing groups, which exhibit high signal to background ratios, have been widely used to image tumors and monitor their growth. A photo-triggered cycloaddition reaction between the arginine-glycine-aspartic acid -N-ɛ-acryllysine (RGD-Acrk) peptides and the tetrazole compounds bound to the surface of biodegradable mesoporous silica nanoparticles (bMSN) has been used to construct a fluorescent nanoprobe (bMSN@T2-RGD-Acrk), which showed fluorescent emission at 550 nm and could selectively image the 4T1 cells and breast cancer. This means that the bMSN@T2-RGD-Acrk nanoprobe made by photo-triggered conjugation approach is a promising fluorescent imaging agent for visualizing tumors. Thus, the photo-triggered one-spot reaction can give a stable crosslinker in a biocompatible manner for bioconjugation with nanoparticles and produce a fluorescent group that is suitable for imaging in vivo.
荧光探针与肽或抗体导向基团结合，具有高信号与背景比，已广泛用于肿瘤成像和监测其生长。一种光触发的精氨酸-甘氨酸-天冬氨酸-N-ɛ-丙烯酰赖氨酰（RGD-Acrk）肽与表面结合的四唑化合物之间的环加成反应被用于构建荧光纳米探针（bMSN@T2-RGD-Acrk），其在 550nm 处具有荧光发射，并且可以选择性地对 4T1 细胞和乳腺癌进行成像。这意味着，通过光触发偶联方法制备的 bMSN@T2-RGD-Acrk 纳米探针是一种有前途的荧光成像剂，可用于可视化肿瘤。因此，光触发的单点反应可以以生物相容的方式提供稳定的交联剂，用于与纳米颗粒进行生物偶联，并产生适合体内成像的荧光基团。
