Jones T C, Alkan S, Erb P
Parasite Immunol. 1987 Jan;9(1):117-31. doi: 10.1111/j.1365-3024.1987.tb00493.x.
Murine toxoplasmosis caused by a low virulence, cyst-forming strain of Toxcoplasma gondii (Pe strain) is characterized by splenomegaly, lymphadenopathy, decreased delayed-type hypersensitivity (DTH), and the presence of toxoplasma cysts in brain tissue. Cyclophosphamide (CY) in a single dose of 100 mg/kg injected 3 days before infection, or splenectomy 3 weeks before infection, augmented DTH and decreased the number of toxoplasma brain cysts. CY-induced augmentation of resistance during the first 3 weeks of murine toxoplasmosis was associated with: (1) an increase in mononuclear phagocytes and a decrease in T lymphocytes (including Lyt2+ cells) in spleens and lymph nodes; (2) suppressed toxoplasma antigen induced proliferation of cultured spleen cells: (3) augmentation of antigen induced proliferation of cultured lymph node cells; and (4) low levels of interferon-gamma production in both spleen and lymph node cultures. The best correlate of the enhanced in-vivo effects of CY was proliferation of nylon wool-purified lymph node cells to toxoplasma antigen. The presence of Lyt2+ cells in lymph nodes of toxoplasma infected mice inhibited maximal proliferation.
由低毒力、形成包囊的刚地弓形虫菌株（Pe株）引起的小鼠弓形虫病的特征为脾肿大、淋巴结病、迟发型超敏反应（DTH）降低以及脑组织中存在弓形虫包囊。在感染前3天注射单剂量100 mg/kg的环磷酰胺（CY），或在感染前3周进行脾切除术，可增强DTH并减少弓形虫脑包囊的数量。CY在小鼠弓形虫病的前3周诱导的抵抗力增强与以下因素有关：（1）脾脏和淋巴结中单核吞噬细胞增加，T淋巴细胞（包括Lyt2+细胞）减少；（2）弓形虫抗原诱导的培养脾细胞增殖受到抑制；（3）抗原诱导的培养淋巴结细胞增殖增强；（4）脾脏和淋巴结培养物中干扰素-γ产生水平较低。CY体内增强作用的最佳相关因素是尼龙毛纯化的淋巴结细胞对弓形虫抗原的增殖。弓形虫感染小鼠淋巴结中Lyt2+细胞的存在抑制了最大增殖。
