Hashimoto Y, Kawachi E, Shudo K, Sekiya T
Nucleic Acids Symp Ser. 1986(17):135-8.
Chemical modification of a plasmid containing the human c-Ha-ras proto-oncogene (pSVMBras-gpt) in vitro with the ultimate carcinogens N-acetoxy-2-amino-6-methyldipyrido[1,2-a: 3',2'-d]imidazole (N-OAc-Glu-P-1) and N-acetoxy-4-aminoquinoline N-oxide (N-OAc-4AQO) generated an activated oncogene that transformed NIH3T3 cells. As DNA is only cellular macromolecule present in the reactions, the results clearly show that the chemical modification of DNA with carcinogens alone can cause the induction of transformation of mammalian cells.
用最终致癌物N-乙酰氧基-2-氨基-6-甲基二吡啶并[1,2-a:3',2'-d]咪唑（N-OAc-Glu-P-1）和N-乙酰氧基-4-氨基喹啉N-氧化物（N-OAc-4AQO）在体外对含有人类c-Ha-ras原癌基因的质粒（pSVMBras-gpt）进行化学修饰，产生了一种激活的癌基因，该基因可转化NIH3T3细胞。由于DNA是反应中仅有的细胞大分子，结果清楚地表明，仅用致癌物对DNA进行化学修饰就能导致哺乳动物细胞转化的诱导。
