College of Veterinary Medicine, South China Agricultural University, Guangzhou, China.
Key Laboratory of Zoonosis Prevention and Control of Guangdong Province, Guangzhou, China.
PLoS One. 2019 May 2;14(5):e0216245. doi: 10.1371/journal.pone.0216245. eCollection 2019.
Senecavirus A (SVA) is a critical pathogen causing vesicular lesions in sows and acute death of newborn piglets, resulting in very large economic losses in the pig industry. To restrict the transmission of SVA, an establishment of an effective diagnostic method is crucial for the prevention and control of the disease. However, traditional detection methods often have many drawbacks. In this study, reverse transcription loop-mediated isothermal amplification (RT-LAMP) was combined with a lateral flow dipstick (LFD) to detect SVA. The resulting RT-LAMP-LFD assay was performed at 60°C for 50 min and then directly judged on an LFD visualization strip. This method shows high specificity and sensitivity to SVA. The detection limit of RT-LAMP was 4.56x10-8 ng/μL RNA, approximately 11 copies/μL RNA, and it was 10 times more sensitive than RT-PCR. This detection method's positive rate for clinical samples is comparable to that of RT-PCR. This method is time saving and highly efficient and is thus expected to be used to diagnose SVA infections in this field.
塞尼卡病毒 A（SVA）是一种重要的病原体，可导致母猪出现水疱病变，并使新生仔猪急性死亡，给养猪业造成巨大的经济损失。为了限制 SVA 的传播，建立一种有效的诊断方法对于疾病的预防和控制至关重要。然而，传统的检测方法往往存在许多缺点。在本研究中，我们将逆转录环介导等温扩增（RT-LAMP）与侧向流动试纸条（LFD）相结合，用于检测 SVA。所得的 RT-LAMP-LFD 检测法在 60°C 下进行 50 分钟，然后直接在 LFD 可视化条上进行判断。该方法对 SVA 具有高度的特异性和敏感性。RT-LAMP 的检测限为 4.56x10-8ng/μL RNA，约为 11 个拷贝/μL RNA，比 RT-PCR 灵敏 10 倍。该检测方法对临床样本的阳性率与 RT-PCR 相当。该方法省时高效，有望用于该领域的 SVA 感染诊断。
