Miao Mingsan, Peng Mengfan, Zhu Zhengwang, Yan Xiaoli, Wei Zhenzhen, Li Mengyan
College of Pharmacy, Henan University of Chinese Medicine, Zhengzhou 450000, China.
Saudi J Biol Sci. 2019 May;26(4):821-827. doi: 10.1016/j.sjbs.2019.02.007. Epub 2019 Feb 19.
Explore the effects of dodder total flavone on polycystic ovary syndrome (PCOS) rat models induced by dehydroepiandrosterone (DHEA) combined human chorionic gonadotropin (HCG).
Except the blank group, the rest of the rats were injected with DHEA 6 mg/100 g on the back of the neck and 1.5 IU HCG each day, for 21 consecutive days. On the 16th day of modeling, vaginal smear was performed to select the model rats, which were randomly divided into model group, dacin-35 group, large, middle and small dose dodder total flavonoids groups, and given the medicine for three weeks. At the end of the last administration, take samples, so as to calculate the ovaries and uterus indexes, measure serum LH/FSH ratio, P, PRL and INS levels, fixed the uterus and pancreas in 10% formalin solution and stained with HE to observe the morphological changes of the organs. And measure the expression of TNF-α and IGF-l proteins in ovaries by immunohistochemistry.
Compared with the blank group, ovarian and uterine indexes, serum LH/FSH ratio, serum PRL and INS levels, ovary TNF-α and IGF-l protein expression were significantly increased, and significant pathological changes were observed in the uterine and pancreatic tissues in model group ( < 0.01). While the serum P level decreased significantly ( < 0.01), Compared with the model group, the ovarian and uterine indexes, serum LH/FSH ratio, serum P, PRL and INS levels, ovary TNF-α protein expression were significantly decreased in large, middle and small dose dodder total flavonoids groups ( < 0.01); The expression of IGF-1 protein was decreased and uterus pathological changes were improved in different extents ( < 0.01 or < 0.05), pancreas pathological changes were improved significantly ( < 0.01).
PCOS rat models was successfully replicated. Dodder total flavone can protect PCOS rats induced by DHEA combined HCG by different action pathways.
探讨菟丝子总黄酮对脱氢表雄酮（DHEA）联合人绒毛膜促性腺激素（HCG）诱导的多囊卵巢综合征（PCOS）大鼠模型的影响。
除空白组外，其余大鼠于颈部皮下注射6 mg/100 g的DHEA，并每日注射1.5 IU的HCG，连续注射21天。造模第16天，行阴道涂片筛选模型大鼠，将其随机分为模型组、达英-35组、菟丝子总黄酮大、中、小剂量组，给药3周。末次给药结束后，取材，计算卵巢及子宫指数，检测血清LH/FSH比值、P、PRL及INS水平，将子宫和胰腺用10%甲醛固定，HE染色观察脏器形态变化。并采用免疫组化法检测卵巢组织中TNF-α和IGF-1蛋白的表达。
与空白组比较，模型组大鼠卵巢及子宫指数、血清LH/FSH比值、血清PRL及INS水平、卵巢TNF-α和IGF-1蛋白表达显著升高，子宫和胰腺组织出现明显病理改变（P＜0.01）；血清P水平显著降低（P＜0.01）。与模型组比较，菟丝子总黄酮大、中、小剂量组大鼠卵巢及子宫指数、血清LH/FSH比值、血清P、PRL及INS水平、卵巢TNF-α蛋白表达显著降低（P＜0.01）；IGF-1蛋白表达降低，子宫病理改变不同程度改善（P＜0.01或P＜0.05），胰腺病理改变显著改善（P＜0.01）。
成功复制PCOS大鼠模型。菟丝子总黄酮可通过不同作用途径对DHEA联合HCG诱导的PCOS大鼠起到保护作用。
