Richert Elisabeth, Bartsch Sofya, Hillenkamp Jost, Treumer Felix, Tode Jan, von der Burchard Claus, Brinkmann Ralf, Klettner Alexa Karina, Roider Johann
Klinik für Ophthalmologie, Universitätsklinikum Schleswig-Holstein, Christian-Albrechts-Universität zu Kiel, Kiel.
Augenklinik und Poliklinik, Universitätsklinikum Würzburg.
Klin Monbl Augenheilkd. 2020 Feb;237(2):192-201. doi: 10.1055/a-0838-5633. Epub 2019 May 2.
To investigate the effect of Selective Retina Therapy (SRT) on inflammatory key factors such as complement factor-C3 (CC3), tumor growth factor-beta2 (TGF-β2), tumor necrosis factor-alpha (TNF-α) and interferon-gamma (IFN-γ).
Porcine RPE-Bruch's membrane-choroid explants were irradiated with two SRT laser systems, SRT and SRT (Nd : YLF laser, wave length 527 nm, pulse duration 1.7 µs and Nd : YAG laser, wave length 532 nm, pulse duration 2.4 - 3 µs). Laser irradiation was performed on a spot size of 200 × 200 µm, 30 pulses, with a repetition rate of 100 Hz, and a radiant exposure of 140 (threshold RPE death) and 180 mJ/cm per pulse (above threshold RPE death). Explants were cultivated in modified Ussing chambers and culture viability was assessed by calcein-AM cell staining. Secretion of inflammatory factors was analyzed by ELISA. Protein expression of tissue explants was assessed by Western blot.
Regeneration of RPE was observed after 4 days. One day after SRT with 140 mJ/cm per pulse the secretion of basal CC3 decreased in ELISA. Following 180 mJ/cm radiant exposure, the level of IFN-γ decreased at day 4.
SRT does not induce the release of the pro-inflammatory factors analyzed in this in-vitro study.
研究选择性视网膜治疗（SRT）对炎症关键因子如补体因子 - C3（CC3）、肿瘤生长因子 - β2（TGF - β2）、肿瘤坏死因子 - α（TNF - α）和干扰素 - γ（IFN - γ）的影响。
用两种SRT激光系统（SRT和SRT（钕：钇锂氟激光，波长527nm，脉冲持续时间1.7µs；钕：钇铝石榴石激光，波长532nm，脉冲持续时间2.4 - 3µs））对猪视网膜色素上皮 - 布鲁赫膜 - 脉络膜外植体进行照射。激光照射光斑尺寸为200×200µm，30个脉冲，重复频率为100Hz，每个脉冲的辐射曝光量为140（视网膜色素上皮细胞死亡阈值）和180mJ/cm（高于视网膜色素上皮细胞死亡阈值）。外植体在改良的乌斯辛氏小室中培养，通过钙黄绿素 - AM细胞染色评估培养活力。通过酶联免疫吸附测定法分析炎症因子的分泌情况。通过蛋白质印迹法评估组织外植体的蛋白质表达。
4天后观察到视网膜色素上皮细胞再生。在每个脉冲140mJ/cm的SRT治疗1天后，酶联免疫吸附测定法显示基础CC3的分泌减少。在180mJ/cm的辐射曝光后，第4天IFN - γ水平下降。
在这项体外研究中，SRT不会诱导所分析的促炎因子的释放。
