Hilburger Claire E, Jacobs Miranda L, Lewis Kamryn R, Peruzzi Justin A, Kamat Neha P
ACS Synth Biol. 2019 Jun 21;8(6):1224-1230. doi: 10.1021/acssynbio.8b00435. Epub 2019 May 14.
The assembly of channel proteins into vesicle membranes is a useful strategy to control activities of vesicle-based systems. Here, we developed a membrane AND gate that responds to both a fatty acid and a pore-forming channel protein to induce the release of encapsulated cargo. We explored how membrane composition affects the functional assembly of α-hemolysin into phospholipid vesicles as a function of oleic acid content and α-hemolysin concentration. We then showed that we could induce α-hemolysin assembly when we added oleic acid micelles to a specific composition of phospholipid vesicles. Finally, we demonstrated that our membrane AND gate could be coupled to a gene expression system. Our study provides a new method to control the temporal dynamics of vesicle permeability by controlling when the functional assembly of a channel protein into synthetic vesicles occurs. Furthermore, a membrane AND gate that utilizes membrane-associating biomolecules introduces a new way to implement Boolean logic that should complement genetic logic circuits and ultimately enhance the capabilities of artificial cellular systems.
将通道蛋白组装到囊泡膜中是控制基于囊泡的系统活性的一种有效策略。在此，我们开发了一种膜与门，它对脂肪酸和形成孔道的通道蛋白都有响应，从而诱导被封装货物的释放。我们探究了膜组成如何作为油酸含量和α - 溶血素浓度的函数影响α - 溶血素在磷脂囊泡中的功能组装。然后我们表明，当向特定组成的磷脂囊泡中添加油酸胶束时，我们能够诱导α - 溶血素组装。最后，我们证明了我们的膜与门可以与基因表达系统偶联。我们的研究提供了一种新方法，通过控制通道蛋白何时在合成囊泡中进行功能组装来控制囊泡通透性的时间动态。此外，利用与膜相关的生物分子的膜与门引入了一种实现布尔逻辑的新方法，这应该会补充遗传逻辑电路并最终增强人工细胞系统的能力。
