Key Laboratory of Eutrophication and Red Tide Prevention of Guangdong Higher Education Institutes, College of Life Science and Technology, Jinan University, Guangzhou 510632, China.
Mar Drugs. 2019 Apr 30;17(5):259. doi: 10.3390/md17050259.
is a typical benthic toxic dinoflagellate, which can produce phycotoxins such as okadaic acid (OA). In this study, we identified three ABC transporter genes (, and ) and characterized their expression patterns, as well as OA production under different environmental conditions in . We found that the three ABC transporters all showed high identity with related ABC proteins from other species, and contained classical features of ABC transport proteins. Among them, was a half size transporter. The three ABC transporter genes displayed various expression profiles under different conditions. The high concentration of Cu could up-regulate , and transcripts in , suggesting the potential defensive role of ABC transporters against metal ions in surrounding waters. Cu, in some concentration, could induce OA production; meanwhile, tributyltin inhibited OA accumulation. The grazer could induce OA production, and displayed some toxicity to the grazer, indicating the possibility of OA as an anti-grazing chemical. Collectively, our results revealed intriguing data about OA production and the expression patterns of three ABC transporter genes. However, we could not find any significant correlation between OA production and expression pattern of the three ABC transporters in . Our results might provide new molecular insights on the defensive responses of to the surrounding environment.
是一种典型的底栖产毒甲藻，能够产生麻痹性贝类毒素（PSP）如 okadaic 酸（OA）。在这项研究中，我们鉴定了三个 ABC 转运体基因（、和），并研究了它们在不同环境条件下的表达模式和 OA 产量。我们发现，这三个 ABC 转运体与其他物种的相关 ABC 蛋白具有高度同源性，并包含 ABC 转运蛋白的典型特征。其中，是一个半尺寸的转运体。三个 ABC 转运体基因在不同条件下表现出不同的表达谱。高浓度的 Cu 可以上调、和在中的转录物，表明 ABC 转运体在应对周围水中金属离子时可能具有防御作用。在某些浓度下，Cu 可以诱导 OA 产生；同时，三丁基锡抑制 OA 积累。食草动物可以诱导 OA 产生，并且对食草动物表现出一定的毒性，表明 OA 作为一种抗食草化学物质的可能性。总的来说，我们的结果揭示了关于 OA 产生和三个 ABC 转运体基因表达模式的有趣数据。然而，我们在中没有发现 OA 产量和三个 ABC 转运体表达模式之间的任何显著相关性。我们的结果可能为提供了关于对周围环境的防御反应的新的分子见解。
