Field Jeffrey J, Squier Jeff A, Bartels Randy A
Opt Express. 2019 Apr 29;27(9):13015-13030. doi: 10.1364/OE.27.013015.
Fluorescence microscopy is a powerful method for producing high fidelity images with high spatial resolution, particularly in the biological sciences. We recently introduced coherent holographic image reconstruction by phase transfer (CHIRPT), a single-pixel imaging method that significantly improves the depth of field in fluorescence microscopy and enables holographic refocusing of fluorescent light. Here we demonstrate that by installing a confocal slit conjugate to the illuminating light sheets used in CHIRPT, out-of-focus light is rejected, thus improving lateral spatial resolution and rejecting noise from out-of-focus fluorescent light. Confocal CHIRPT is demonstrated and fully modeled. Finally, we explore the use of beam shaping and point-spread-function engineering to enable holographic single-lens light-sheet microscopy with single-pixel detection.
荧光显微镜是一种强大的方法，可用于生成具有高空间分辨率的高保真图像，尤其是在生物科学领域。我们最近引入了通过相位转移进行相干全息图像重建（CHIRPT），这是一种单像素成像方法，可显著提高荧光显微镜的景深，并实现荧光光的全息重新聚焦。在这里，我们证明通过安装与CHIRPT中使用的照明光片共轭的共焦狭缝，可以排除离焦光，从而提高横向空间分辨率并排除来自离焦荧光光的噪声。展示并对共焦CHIRPT进行了完整建模。最后，我们探索了使用光束整形和点扩散函数工程来实现具有单像素检测的全息单透镜光片显微镜。
