Finding the peak of dynamic oxygen uptake during fatiguing exercise in fish.

As fish approach fatigue at high water velocities in a critical swimming speed () test, their swimming mode and oxygen cascade typically move to an unsteady state because they adopt an unsteady, burst-and-glide swimming mode despite a constant, imposed workload. However, conventional rate of oxygen uptake ( ) sampling intervals (5-20 min) tend to smooth any dynamic fluctuations in active  () and thus likely underestimate the peak Here, we used rainbow trout () to explore the dynamic nature of near using various sampling windows and an iterative algorithm. Compared with a conventional interval regression analysis of over a 10-min period, our new analytical approach generated a 23% higher peak Therefore, we suggest that accounting for such dynamics in with this new analytical approach may lead to more accurate estimates of maximum  in fishes.