Department of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran.
Department of Chemistry, Mashhad Branch, Islamic Azad University, Mashhad, Iran.
IET Nanobiotechnol. 2019 May;13(3):269-274. doi: 10.1049/iet-nbt.2018.5190.
Cancer is one of the leading causes of human death. Nanotechnology could offer new and optimised anticancer agents in order to fight cancer. It was shown that metal nanoparticles, in particular silver nanoparticles (AgNPs) were effective in cancer therapy. In this study, AgNPs were synthesised using L. extract (Ru-AgNPs). Then, cytotoxicity effects of the Ru-AgNPs against MDA-MB-231 carcinoma cell line and human dermal fibroblast as normal cell line were performed. Furthermore, anti-apoptotic effects of Ru-AgNPs on these cancer and normal cell lines were compared using acridine orange/propidium iodide staining, flow cytometry analysis and real-time qPCR in apoptosis gene markers. Results of UV-vis spectroscopy showed that Ru-AgNPs have a peak at 430 nm, which indicated synthesis of AgNPs. Ru-AgNPs had spherical shape and average size of 12 nm. Ru-AgNPs have cytotoxicity on MDA-MB-231 cells and decrease cancerous cell viability (IC50 = 4 µg/ml/48 h). Ru-AgNPs could induce apoptosis in MDA-MB-231 cells through upregulation of Bax and downregulation of Bcl-2 gene expression. The results opened up new avenues to develop Rubia based metal complexes as an anticancer agent.
癌症是人类死亡的主要原因之一。纳米技术可以提供新的和优化的抗癌药物，以对抗癌症。研究表明，金属纳米粒子，特别是银纳米粒子（AgNPs）在癌症治疗中非常有效。在这项研究中，使用 L. extract（Ru-AgNPs）合成了 AgNPs。然后，研究了 Ru-AgNPs 对 MDA-MB-231 癌细胞系和人真皮成纤维细胞（正常细胞系）的细胞毒性作用。此外，通过吖啶橙/碘化丙啶染色、流式细胞术分析和实时 qPCR 在凋亡基因标志物中比较了 Ru-AgNPs 对这些癌细胞和正常细胞系的抗凋亡作用。紫外-可见光谱结果表明，Ru-AgNPs 在 430nm 处有一个峰值，这表明 AgNPs 的合成。Ru-AgNPs 呈球形，平均粒径为 12nm。Ru-AgNPs 对 MDA-MB-231 细胞具有细胞毒性，并降低癌细胞活力（IC50=4μg/ml/48h）。Ru-AgNPs 可以通过上调 Bax 和下调 Bcl-2 基因表达诱导 MDA-MB-231 细胞凋亡。这些结果为开发基于 Rubia 的金属配合物作为抗癌药物开辟了新途径。
