School of Life Sciences, Jaipur National University, Jaipur, India.
Department of Community Medicine & School of Public Health, Postgraduate Institute of Medical Education and Research (PGIMER), Chandigarh, India.
Anticancer Agents Med Chem. 2021;21(10):1266-1274. doi: 10.2174/1871520620999201001201230.
There is an urgent need to devise improved alternatives for the efficient delivery of drugs to develop improved therapeutic interventions against cancers. Nanotechnology-based drug delivery vehicles are in-use with obvious issues of toxicity and bio-distribution. Therefore, green synthetic routes are being deployed to replace the conventional nanoparticle formulations for effective drug delivery aiming at developing interventional strategies against cancer.
A simple, viable, and fast approach was used for the green synthesis of silver nanoparticles (AgNPs) using aqueous leaf-extract of Viburnum nervosum (VN) and to explore the anti-cancer potential of the crude extract of VN.
Silver NPs were synthesized by reacting silver nitrate (AgNO) with leaf extract of VN. Various analytical techniques were used to characterize the AgNPs. Finally, the anti-cancer potential of VN was observed when delivered through AgNPs.
The surface plasmon spectra for AgNPs exhibited absorbance peak at 445 nm, and Fourier-Transform Infrared Spectroscopy investigation revealed the presence of biomolecules acting as an effective reducing and capping agent for converting silver nitrate to AgNPs. Further, our results suggest the spherical size of synthesized AgNPs ranging from 12-17 nm. Moreover, in vitro studies conducted for VN extract with breast (MCF-7) and epidermal carcinoma (A431) cells showed biocompatibility.
Doxorubicin loaded AgNPs documented an increased bioavailability of the drug compared to the free drug, suggesting the use of AgNPs as "novel drug delivery vectors".
迫切需要设计改进的药物输送方法，以开发针对癌症的改良治疗干预措施。基于纳米技术的药物输送载体已在使用，但存在毒性和生物分布等明显问题。因此，正在采用绿色合成路线来替代常规的纳米颗粒制剂，以实现有效的药物输送，旨在开发针对癌症的介入策略。
使用 Viburnum nervosum（VN）的水提叶提取物，采用简单、可行且快速的方法绿色合成银纳米粒子（AgNPs），并探索 VN 粗提物的抗癌潜力。
通过将硝酸银（AgNO）与 VN 的叶提取物反应来合成 AgNPs。采用各种分析技术对 AgNPs 进行了表征。最后，通过 AgNPs 输送观察 VN 的抗癌潜力。
AgNPs 的表面等离子体光谱在 445nm 处显示出吸收峰，傅里叶变换红外光谱研究表明存在生物分子，它们作为有效的还原剂和封端剂，将硝酸银转化为 AgNPs。此外，我们的结果表明合成的 AgNPs 的球形尺寸在 12-17nm 之间。此外，VN 提取物对乳腺（MCF-7）和表皮癌细胞（A431）的体外研究表明其具有生物相容性。
与游离药物相比，载多柔比星的 AgNPs 记录了药物的生物利用度增加，表明 AgNPs 可作为“新型药物输送载体”。
