Institute of Biochemistry and Biophysics (IBB), University of Tehran, Tehran, Iran.
Department of Chemistry, University of Oslo, Oslo, Norway.
Sci Rep. 2017 Jul 12;7(1):5178. doi: 10.1038/s41598-017-05461-3.
With the aim of making specific targeting of silver nanoparticles as a drug for tumor cells and developing new anticancer agents, a novel nano-composite was developed. Albumin coated silver nanoparticles (ASNPs) were synthesized, and their anti-cancerous effects were evaluated against MDA-MB 231, a human breast cancer cell line. The synthesized ASNPs were characterized by spectroscopic methods. The morphological changes of the cells were observed by inverted, florescent microscopy and also by DNA ladder pattern on gel electrophoresis; the results revealed that the cell death process occurred through the apoptosis mechanism. It was found that ASNPs with a size of 90 nm and negatively charged with a zeta-potential of about -20 mV could be specifically taken up by tumor cells. The LD of ASNPs against MDA-MB 231 (5 μM), was found to be 30 times higher than that for white normal blood cells (152 μM). The characteristics of the synthesized ASNPs included; intact structure of coated albumin, higher cytotoxicity against cancer cells than over normal cells, and cell death based on apoptosis and reduction of gland tumor sizes in mice. This work indicates that ASNPs could be a good candidate for chemotherapeutic drug.
为了实现将银纳米粒子作为肿瘤细胞药物的靶向特异性，并开发新的抗癌药物，我们开发了一种新型的纳米复合材料。我们合成了白蛋白包裹的银纳米粒子（ASNPs），并评估了它们对 MDA-MB 231（一种人乳腺癌细胞系）的抗癌作用。通过光谱方法对合成的 ASNPs 进行了表征。通过倒置荧光显微镜观察细胞的形态变化，通过凝胶电泳观察 DNA 梯状图案；结果表明，细胞死亡过程是通过细胞凋亡机制发生的。结果发现，尺寸为 90nm 且带负电荷、Zeta 电位约为-20mV 的 ASNPs 可以被肿瘤细胞特异性摄取。ASNPs 对 MDA-MB 231 的 LD（5μM）比正常白细胞（152μM）高 30 倍。合成的 ASNPs 的特点包括：包裹的白蛋白结构完整、对癌细胞的细胞毒性高于正常细胞、基于细胞凋亡的细胞死亡以及减少小鼠的腺体肿瘤大小。这项工作表明，ASNPs 可能是一种有前途的化疗药物候选物。
