评价银和硫化银纳米颗粒对革兰氏阳性菌和革兰氏阴性菌的毒性。
Evaluation of the toxicities of silver and silver sulfide nanoparticles against Gram-positive and Gram-negative bacteria.
机构信息
Department of Chemistry, School of Chemical and Biotechnology, SASTRA University, Thanjavur 613 401, Tamil Nadu, India.
Department of Chemistry, SRM University, Kattankulathur, Chennai, Tamil Nadu, India.
出版信息
IET Nanobiotechnol. 2019 May;13(3):326-331. doi: 10.1049/iet-nbt.2018.5221.
Abstract
In this study, the endogenous lipid signalling molecules, -myristoylethanolamine, were explored as a capping agent to synthesise stable silver nanoparticles (AgNPs) and Ag sulphide NPs (AgS NPs). Sulphidation of the AgNPs abolishes the surface plasmon resonance (SPR) maximum of AgNPs at 415 nm with concomitant changes in the SPR, indicating the formation of AgS NPs. Transmission electron microscopy revealed that the AgNPs and AgS NPs are spherical in shape with a size of 5-30 and 8-30 nm, respectively. AgNPs and AgS NPs exhibit antimicrobial activity against Gram-positive and Gram-negative bacteria. The minimum inhibitory concentrations (MIC) of 25 and 50 μM for AgNPs and AgS NPs, respectively, were determined from resazurin microtitre plate assay. At or above MIC, both AgNPs and AgS NPs decrease the cell viability through the mechanism of membrane damage and generation of excess reactive oxygen species.
摘要
在这项研究中，内源性脂质信号分子肉豆蔻酰乙醇胺被探索用作稳定剂来合成稳定的银纳米粒子（AgNPs）和银硫化物纳米粒子（AgS NPs）。AgNPs 的硫化会使 AgNPs 在 415nm 处的表面等离子体共振（SPR）最大值消失，同时 SPR 发生变化，表明 AgS NPs 的形成。透射电子显微镜显示，AgNPs 和 AgS NPs 呈球形，尺寸分别为 5-30nm 和 8-30nm。AgNPs 和 AgS NPs 对革兰氏阳性菌和革兰氏阴性菌均具有抗菌活性。通过 Resazurin 微量板测定法，确定 AgNPs 和 AgS NPs 的最小抑菌浓度（MIC）分别为 25 和 50μM。在 MIC 或以上，AgNPs 和 AgS NPs 通过破坏细胞膜和产生过量活性氧物质的机制降低细胞活力。
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