Laboratory of Biosensors, FSBIS G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, pr. Nauki, 5, Pushchino, Moscow Region, 142290, Russian Federation.
IET Nanobiotechnol. 2019 May;13(3):332-338. doi: 10.1049/iet-nbt.2018.5172.
This work considers the effects of various carbon nanomaterials and fibres on bioelectrocatalytic and respiratory activity of bacterial cells during the oxidation of ethanol in the presence of an electron transport mediator. sbsp. VKM B-1280 cells were immobilised on the surfaces of graphite electrodes and had an adsorption contact with a nanomaterial (multi-walled carbon nanotubes, thermally expanded graphite, highly oriented pyrolytic graphite, graphene oxide, reduced graphene oxide). The electrochemical parameters of the electrodes (the polarisation curves, the value of generated current at the introduction of substrate, the impedance characteristics) were measured in two-electrode configuration. Modification by multi-walled carbon nanotubes led to the increase of microbial fuel cell (MFC) electric power by 26%. The charge transfer resistance of modified electrodes was 47% lower than unmodified ones. Thermally expanded and pyrolytic graphites had a slight negative effect on the electrochemical properties of modified electrodes. The respiratory activity of bacterial cells did not change in the presence of nanomaterials. The data can be used in the development of microbial biosensors and MFC electrodes based on cells.
这项工作研究了在电子传输介质存在下，各种碳纳米材料和纤维对细菌细胞在乙醇氧化过程中生物电化学和呼吸活性的影响。sbsp. VKM B-1280 细胞被固定在石墨电极表面，并与纳米材料（多壁碳纳米管、热膨胀石墨、高取向热解石墨、氧化石墨烯、还原氧化石墨烯）具有吸附接触。在两电极配置中测量了电极的电化学参数（极化曲线、引入底物时产生的电流值、阻抗特性）。多壁碳纳米管的修饰导致微生物燃料电池（MFC）的电功率增加了 26%。修饰电极的电荷转移电阻比未修饰电极低 47%。热膨胀石墨和热解石墨对修饰电极的电化学性能有轻微的负面影响。在纳米材料存在的情况下，细菌细胞的呼吸活性没有改变。这些数据可用于基于细胞的微生物生物传感器和 MFC 电极的开发。
