Department of Biochemistry, Genetics and Microbiology, Forestry and Agricultural Biotechnology Institute (FABI), University of Pretoria, Pretoria, Gauteng, 0002, South Africa.
Agricultural Research Council (ARC), Biotechnology Platform, Onderstepoort, Gauteng, 0110, South Africa.
Antonie Van Leeuwenhoek. 2019 Sep;112(9):1369-1385. doi: 10.1007/s10482-019-01269-5. Epub 2019 May 3.
Twelve nodulating Paraburkholderia strains isolated from indigenous South African fynbos legume Hypocalyptus sophoroides were investigated to determine their taxonomic status. Genealogical concordance analysis, based on six loci (16S rRNA, atpD, recA, rpoB, lepA and gltB), revealed that they separate into two consistent and exclusive groups. Average nucleotide identity and DNA-DNA hybridisation comparisons indicated that they were sufficiently divergent from their closest known phylogenetic relatives (Paraburkholderia caledonica and Paraburkholderia terrae, respectively) to be regarded as novel species. This was also supported by the results of fatty acid analysis and metabolic characterisation. For these two isolate groups, we accordingly propose the new species Paraburkholderia strydomiana sp. nov. with WK1.1f (= LMG 28731 = SARCC1213) as its type strain and Paraburkholderia steynii sp. nov. with HC1.1ba (= LMG 28730 = SARCC696) as its type strain. Our data thus showed that H. sophoroides may be considered a promiscuous symbiotic partner due to its ability to associate with multiple species of Paraburkholderia.
从南非本土的法尔布森林豆科植物 Hypocalyptus sophoroides 中分离出的 12 株结瘤 Paraburkholderia 菌株被用于确定它们的分类地位。基于 6 个基因座（16S rRNA、atpD、recA、rpoB、lepA 和 gltB）的系统发育一致性分析表明，它们分为两个一致且独特的群。平均核苷酸同一性和 DNA-DNA 杂交比较表明，它们与最近的已知系统发育近亲（分别为 Paraburkholderia caledonica 和 Paraburkholderia terrae）足够分化，可被视为新种。脂肪酸分析和代谢特征的结果也支持了这一点。对于这两个分离株群，我们相应地提出了新种 Paraburkholderia strydomiana sp. nov.，其模式菌株为 WK1.1f（= LMG 28731 = SARCC1213），以及 Paraburkholderia steynii sp. nov.，其模式菌株为 HC1.1ba（= LMG 28730 = SARCC696）。我们的数据表明，由于 H. sophoroides 能够与多种 Paraburkholderia 物种相关联，因此可以将其视为一种混杂的共生伙伴。
