Bournaud Caroline, Moulin Lionel, Cnockaert Margo, Faria Sergio de, Prin Yves, Severac Dany, Vandamme Peter
Present address: Embrapa Genetic Resources and Biotechnology, Pest-Plant Molecular Interaction Laboratory, Brasilia, Distrito Federal, Brazil.
Cirad, IRD, Univ. Montpellier, SupAgro, Laboratoire des Symbioses Tropicales et Méditerranéennes, 34398 Montpellier, France.
Int J Syst Evol Microbiol. 2017 Feb;67(2):432-440. doi: 10.1099/ijsem.0.001648. Epub 2017 Mar 16.
During a survey of root-nodulating symbionts of Mimosoid species in the south-east region of Brazil, eight Paraburkholderia isolates were obtained from nodules of the legume species Piptadenia gonoacantha, either from the field or following a soil trapping method with the same plant host. 16S rRNA gene as well as recA and gyrB phylogenetic markers placed these strains in two new clades within the genus Burkholderia sensu lato. DNA-DNA hybridization values and analyses of average nucleotide identities of the whole genome sequence of selected strains in each clade (STM 7183 and STM 7296) showed that the two clades represented novel species of the genus Paraburkholderia. All eight isolates were further characterized using DNA base content determination, chemotaxonomic and biochemical profiling and symbiotic properties, which allowed to distinguish the novel species from known diazotrophic species of the genus Paraburkholderia. Based on genomic and phenotypic data, the names Paraburkholderia piptadeniae sp. nov. with type strain STM 7183T (=DSM 101189T=LMG 29163T) and Paraburkholderia ribeironis sp. nov. with type strain STM 7296T (=DSM 101188T=LMG 29351T) are proposed.
在对巴西东南部含羞草科物种的根瘤共生体进行调查期间，从豆科植物贡氏腺荚豆的根瘤中获得了8株类伯克霍尔德氏菌分离株，这些分离株有的采自田间，有的是采用相同植物宿主的土壤诱捕法获得的。16S rRNA基因以及recA和gyrB系统发育标记将这些菌株置于广义伯克霍尔德氏菌属内的两个新分支中。每个分支中选定菌株（STM 7183和STM 7296）的DNA-DNA杂交值以及全基因组序列的平均核苷酸同一性分析表明，这两个分支代表了类伯克霍尔德氏菌属的新物种。使用DNA碱基含量测定、化学分类学和生化分析以及共生特性对所有8株分离株进行了进一步表征，从而能够将这些新物种与类伯克霍尔德氏菌属已知的固氮物种区分开来。基于基因组和表型数据，提出了新物种贡氏类伯克霍尔德氏菌（Paraburkholderia piptadeniae sp. nov.），模式菌株为STM 7183T（=DSM 101189T=LMG 29163T），以及新物种里贝罗类伯克霍尔德氏菌（Paraburkholderia ribeironis sp. nov.），模式菌株为STM 7296T（=DSM 101188T=LMG 29351T）。
