Department of Biochemistry, Genetics and Microbiology, Forestry and Agricultural Biotechnology Institute (FABI), University of Pretoria, Pretoria, South Africa.
Department of Biochemistry, Genetics and Microbiology, Forestry and Agricultural Biotechnology Institute (FABI), University of Pretoria, Pretoria, South Africa; School of Life Sciences, University of Nevada Las Vegas, Las Vegas, NV, United States of America.
Syst Appl Microbiol. 2021 Jan;44(1):126152. doi: 10.1016/j.syapm.2020.126152. Epub 2020 Oct 28.
Previous studies have recognized South and Central/Latin American mimosoid legumes in the genera Mimosa, Piptadenia and Calliandra as hosts for various nodulating Paraburkholderia species. Several of these species have been validly named in the last two decades, e.g., P. nodosa, P. phymatum, P. diazotrophica, P. piptadeniae, P. ribeironis, P. sabiae and P. mimosarum. There are still, however, a number of diverse Paraburkholderia strains associated with these legumes that have an unclear taxonomic status. In this study, we focus on 30 of these strains which originate from the root nodules of Brazilian and Mexican Mimosa species. They were initially identified as P. tuberum and subsequently placed into a symbiovar (sv. mimosae) based on their host preferences. A polyphasic approach for the delineation of these strains was used, consisting of genealogical concordance analysis (using atpD, gyrB, acnA, pab and 16S rRNA gene sequences), together with comparisons of Average Nucleotide Identity (ANI), DNA G+C content ratios and phenotypic characteristics with those of the type strains of validly named Paraburkholderia species. Accordingly, these 30 strains were delineated into two distinct groups, of which one is conspecific with 'P. atlantica' CNPSo 3155 and the other new to Science. We propose the name Paraburkholderia youngii sp. nov. with type strain JPY169 (= LMG 31411; SARCC751) for this novel species.
先前的研究已经将南美的含羞草科植物和中美的金合欢科植物中的某些属（如含羞草属、金合欢属和Calliandra 属）的植物鉴定为各种根瘤菌属（Paraburkholderia）的宿主。在过去的二十年中，已经对其中的一些物种进行了有效命名，例如，P. nodosa、P. phymatum、P. diazotrophica、P. piptadeniae、P. ribeironis、P. sabiae 和 P. mimosarum。然而，与这些豆科植物相关的仍然有许多不同的根瘤菌菌株，它们的分类地位尚不清楚。在这项研究中，我们关注的是来自巴西和墨西哥含羞草属植物根瘤的 30 株此类菌株。它们最初被鉴定为 P. tuberum，随后根据其宿主偏好被归入一个共生群（sv. mimosae）。使用多相方法来划分这些菌株，包括系统发育一致性分析（使用 atpD、gyrB、acnA、pab 和 16S rRNA 基因序列），并与有效命名的根瘤菌属种的标准菌株进行平均核苷酸同一性（ANI）、DNA G+C 含量比值和表型特征的比较。因此，这 30 株菌株被划分为两个不同的群体，其中一个与“P. atlantica”CNPSo 3155 相同，另一个则是新的科学分类。我们建议将这种新的物种命名为 Paraburkholderia youngii sp. nov.，其模式菌株为 JPY169（= LMG 31411；SARCC751）。
