Department of Pharmaceutics & Pharmaceutical Technology, Faculty of Pharmacy & Drug Manufacturing, Pharos University in Alexandria, Alexandria, Egypt.
Department of Pharmacology & Therapeutics, Faculty of Pharmacy & Drug Manufacturing, Pharos University in Alexandria, Alexandria, Egypt.
Int J Pharm. 2019 Jun 30;565:70-82. doi: 10.1016/j.ijpharm.2019.04.081. Epub 2019 May 2.
Post-operative adhesion is a common cause of several complications including intestinal obstruction, chronic pelvic pain and/or infertility. Adhesions are fibrous bands that result from the inflammatory reactions due to peritoneum damage. The current study focused on designing an effective anti-inflammatory loaded barrier for the prevention of post-operative adhesions. The proposed method is based on the use of polyvinyl alcohol (PVA), cryobarrier loaded with Ibuprofen (Ibu). Anti-adhesive Ibu-cryobarriers were prepared in different forms, and subjected to in-vitro evaluation comprising; drug release rate, maximum swelling index, morphological examination using scanning electron microscope (SEM), fourier-transform infrared spectroscopy (FTIR) and mechanical properties. Optimized cryobarriers were further investigated for their in-vivo effectiveness in preventing post-operative adhesions in female Sprague-Dawley rats. All formulations showed appropriate physical and morphological characteristics, in-vitro controlled sustained drug release profiles during a period of seven days with acceptable maximum swelling index. Invivo, all cryobarriers were equivalent to each other concerning serum or tissue parameter. However, morphological and histopathological evaluations revealed that both xerocryogel and lyophilized cryofilms are more effective than the cryogel in prevention of post-operative peritoneal adhesions. The current study showed the possibility of preparing drug loaded cryobarriers using simple technique with an effective in vivo post-operative adhesion prevention.
术后粘连是多种并发症的常见原因，包括肠梗阻、慢性盆腔痛和/或不孕。粘连是由于腹膜损伤引起的炎症反应而产生的纤维带。本研究旨在设计一种有效的抗炎负载屏障，以预防术后粘连。该方法基于使用聚乙烯醇（PVA）和负载布洛芬（Ibu）的冰晶屏障。制备了不同形式的抗粘连 Ibu-冰晶屏障，并进行了体外评估，包括药物释放率、最大溶胀指数、扫描电子显微镜（SEM）形态检查、傅里叶变换红外光谱（FTIR）和机械性能。进一步研究了优化的冰晶屏障在预防雌性 Sprague-Dawley 大鼠术后粘连方面的体内有效性。所有制剂均表现出适当的物理和形态特征，在体外可控制药物在七天内持续释放，最大溶胀指数可接受。在体内，所有冰晶屏障在血清或组织参数方面彼此等效。然而，形态学和组织病理学评估表明，干冰晶和冻干冰晶膜在预防术后腹膜粘连方面比冰晶凝胶更有效。本研究表明，使用简单的技术制备负载药物的冰晶屏障具有预防术后粘连的有效性。
