Department of Medical Nanotechnology, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran.
Zanjan Metabolic Diseases Research Center, Zanjan University of Medical Sciences, Zanjan, Iran.
Artif Cells Nanomed Biotechnol. 2022 Dec;50(1):40-48. doi: 10.1080/21691401.2021.1883043.
Adhesion bands are pathological fibrous tissues that create in the middle of tissues and organs, often reasons of intestinal obstruction, and female infertility. Here, we explored the anti-adhesive and inflammatory capacities of PEG/silk and Ibuprofen-loaded PEG/Silk core-shell nanofibrous membranes, respectively. The ibuprofen-loaded Silk Fibroin-Poly ethylene Glycol (SF-PEG) core-shell membrane was fabricated by electrospinning and considered in terms of morphology, surface wettability, drug release, and degradation. To reveal the membrane capability for adhesion bands inhibition, the membrane was stitched among the abdominal partition and peritoneum and then evaluated using two scoring adhesion systems. According to results, the fibrous membrane hindered cell proliferation, and the scoring systems and pathology showed that in a rat model, Ibuprofen-loaded PEG/Silk core-shell membrane caused a lightening in post-operative adhesion bands and the low-grade inflammatory reaction in animal models. Collectively, we fabricated new ibuprofen-loaded PEG/SF membranes with anti-adhesion and anti-inflammation properties. Moreover, this core-shell electrospun fibrous membrane has not even now been used to prevent peritendinous adhesion generation.
粘连带是一种病理性纤维组织，存在于组织和器官的中间，常导致肠梗阻和女性不孕。在这里，我们分别研究了 PEG/丝素和载布洛芬的 PEG/丝素核壳纳米纤维膜的抗粘连和抗炎能力。载布洛芬的丝素蛋白-聚乙二醇（SF-PEG）核壳膜通过静电纺丝制备，并从形态、表面润湿性、药物释放和降解等方面进行了考虑。为了揭示膜对粘连带抑制的能力，将膜缝合在腹部隔板和腹膜之间，然后使用两种评分粘连系统进行评估。结果表明，纤维膜抑制了细胞增殖，评分系统和病理学显示，在大鼠模型中，载布洛芬的 PEG/丝素核壳膜减轻了术后粘连带的形成，并减轻了动物模型中的低级别炎症反应。总之，我们制备了具有抗粘连和抗炎特性的新型载布洛芬的 PEG/SF 膜。此外，这种核壳电纺纤维膜甚至尚未用于预防肌腱周围粘连的产生。
