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中度嗜盐海洋细菌 Marinobacter litoralis SW-45 的全基因组序列:非编码 RNA 的丰度。

Whole genome sequence of moderate halophilic marine bacterium Marinobacter litoralis SW-45: Abundance of non-coding RNAs.

机构信息

School of Bioprocess Engineering, Universiti Malaysia Perlis UNIMAP, Kompleks Pusat Pengajian Jejawi 3, Arau, Perlis, 02600, Malaysia; Centre of Excellence for Biomass Utilization, School of Bioprocess Engineering, Universiti Malaysia Perlis, Kompleks Pusat Pengajian Jejawi 3, Arau, Perlis, 02600 Malaysia.

Centre of Excellence for Biomass Utilization, School of Bioprocess Engineering, Universiti Malaysia Perlis, Kompleks Pusat Pengajian Jejawi 3, Arau, Perlis, 02600 Malaysia; Department of Chemical Engineering Technology, Faculty of Engineering Technology, Universiti Malaysia Perlis, Kampus UniCITI Alam, Sungai Chuchuh, Padang Besar 02100, Perlis, Malaysia.

出版信息

Int J Biol Macromol. 2019 Jul 15;133:1288-1298. doi: 10.1016/j.ijbiomac.2019.05.003. Epub 2019 May 2.

Abstract

A report on the de novo Whole Genome Sequence (WGS) of Marinobacter litoralis SW-45, a moderately salt-tolerant bacterium isolated from the seawater in Malaysia is presented. The strain has a genome size of 3.45 Mb and is capable of producing halophilic lipase, protease and esterase enzymes. Computational prediction of non-coding RNA (ncRNA) genes in M. litoralis SW-45 was performed using standalone software known as the non-coding RNA characterization (nocoRNAc). In addition, a phylogenetic tree showing the evolutionary relationship between the strain and other members of the genus Marinobacter was constructed using 16SrRNA sequence information. A total of 385 ncRNA transcripts, 1124 terminator region, and 2350 Stress Induced Duplex Destabilization sites were predicted. The current WGS shotgun project has provided the relevant genetic information that may be useful for the strain's improvement studies. This manuscript gives the first description of M. litoralis with a complete genome.

摘要

本文报告了从马来西亚海水中分离到的中度耐盐菌 Marinobacter litoralis SW-45 的从头全基因组序列(WGS)。该菌株的基因组大小为 3.45Mb,能够产生嗜盐脂肪酶、蛋白酶和酯酶。使用独立的非编码 RNA 特征分析软件(nocoRNAc)对 M. litoralis SW-45 中的非编码 RNA(ncRNA)基因进行了计算预测。此外,还根据 16SrRNA 序列信息构建了显示该菌株与 Marinobacter 属其他成员之间进化关系的系统发育树。共预测了 385 个 ncRNA 转录物、1124 个终止子区域和 2350 个应激诱导的双链不稳定位点。目前的 WGS 鸟枪法项目提供了相关的遗传信息,这可能对该菌株的改良研究有用。本文首次对完整基因组的 Marinobacter litoralis 进行了描述。

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