Xu Wenjun, Sun Jinru, Le Yangjing, Chen Jingliang, Lu Xiaoyun, Yao Xueling
a State Key Laboratory of Electrical Insulation and Power Equipment , Xi'an Jiaotong University , Xi'an , China.
b School of Life and Engineering , Xi'an Jiaotong University , Xi'an , China.
Electromagn Biol Med. 2019;38(3):185-197. doi: 10.1080/15368378.2019.1608233. Epub 2019 May 6.
In recent years, using electromagnetic fields as a targeted therapy for tumors has become a new idea. This paper aims to study the response of rat glioma cells (C6) when the external electromagnetic field parameters change and to obtain a complete working range of magnetic field parameters. Four-day, 4-h daily millisecond magnetic field exposure experiments were performed with C6 cells. The peak values of magnetic field intensity were 260 mT, 90 mT, 19 mT and 6 mT. Each day after exposure, cell morphology and cell viability assay (MTT method) were measured. The response of C6 cells shows a significant window effect and time cumulative effect on the cell, and it is non-destructive. The working inhibited magnetic field range of magnetic field increase rate d/d (T/s) is [34, 119.5] and [166.75, 527.25], the magnetic field amplitude (mT) is [6, 260], the magnetic field integral (mT·s) is [0.1649, 0.8085] and the energy integral (mT·s) is [2.317, 53.328]. Our findings provide the theoretical and experimental basis for clinical applications of electromagnetic fields.
近年来，利用电磁场作为肿瘤的靶向治疗手段已成为一种新的思路。本文旨在研究大鼠胶质瘤细胞（C6）在外部电磁场参数变化时的反应，并获得磁场参数的完整工作范围。对C6细胞进行了为期四天、每天4小时的毫秒级磁场暴露实验。磁场强度峰值分别为260 mT、90 mT、19 mT和6 mT。暴露后每天测量细胞形态和细胞活力测定（MTT法）。C6细胞的反应对细胞表现出显著的窗口效应和时间累积效应，且具有非破坏性。磁场增加率d/d（T/s）的工作抑制磁场范围为[34, 119.5]和[166.75, 527.25]，磁场幅度（mT）为[6, 260]，磁场积分（mT·s）为[0.1649, 0.8085]，能量积分（mT·s）为[2.317, 53.328]。我们的研究结果为电磁场的临床应用提供了理论和实验依据。
