Trimboli Francesca, Morittu Valeria Maria, Cicino Caterina, Palmieri Camillo, Britti Domenico
Department of Health Science, Magna Græcia University of Catanzaro, Catanzaro 88100, Italy; Interdepartmental Services Centre of Veterinary for Human and Animal Health, Magna Græcia University of Catanzaro, Catanzaro 88100, Italy.
Department of Health Science, Magna Græcia University of Catanzaro, Catanzaro 88100, Italy; Interdepartmental Services Centre of Veterinary for Human and Animal Health, Magna Græcia University of Catanzaro, Catanzaro 88100, Italy.
J Chromatogr A. 2017 Oct 13;1519:131-136. doi: 10.1016/j.chroma.2017.08.075. Epub 2017 Aug 30.
The substitution of ewe milk with more economic cow milk is a common fraud. Here we present a capillary electrophoresis method for the quantification of ewe milk in ovine/bovine milk mixtures, which allows for the rapid and inexpensive recognition of ewe milk adulteration with cow milk. We utilized a routine CE method for human blood and urine proteins analysis, which fulfilled the separation of skimmed milk proteins in alkaline buffer. Under this condition, ovine and bovine milk exhibited a recognizable and distinct CE protein profiles, with a specific ewe peak showing a reproducible migration zone in ovine/bovine mixtures. Based on ewe specific CE peak, we developed a method for ewe milk quantification in ovine/bovine skimmed milk mixtures, which showed good linearity, precision and accuracy, and a minimum amount of detectable fraudulent cow milk equal to 5%.
用更经济的牛奶替代羊奶是一种常见的欺诈行为。在此，我们提出一种毛细管电泳方法，用于定量分析绵羊奶/牛奶混合物中的羊奶，该方法能够快速且低成本地识别羊奶中掺入牛奶的情况。我们采用了一种用于分析人血和尿液蛋白质的常规毛细管电泳方法，该方法可在碱性缓冲液中实现脱脂乳蛋白质的分离。在此条件下，绵羊奶和牛奶呈现出可识别且不同的毛细管电泳蛋白质图谱，在绵羊奶/牛奶混合物中，特定的羊奶峰显示出可重复的迁移区域。基于羊奶特定的毛细管电泳峰，我们开发了一种用于定量分析绵羊奶/牛奶脱脂混合物中羊奶含量的方法，该方法具有良好的线性、精密度和准确度，可检测到的最低掺假牛奶量为5%。
