Spina Anna Antonella, Ceniti Carlotta, Piras Cristian, Tilocca Bruno, Britti Domenico, Morittu Valeria Maria
Interdepartmental Services Centre of Veterinary for Human and Animal Health, Department of Health Science, Magna Græcia University, Catanzaro 88100, Italy.
J Anim Sci Technol. 2022 May;64(3):531-538. doi: 10.5187/jast.2022.e22. Epub 2022 May 31.
In Italy, buffalo mozzarella is a largely sold and consumed dairy product. The fraudulent adulteration of buffalo milk with cheaper and more available milk of other species is very frequent. In the present study, Fourier transform infrared spectroscopy (FTIR), in combination with multivariate analysis by partial least square (PLS) regression, was applied to quantitatively detect the adulteration of buffalo milk with cow milk by using a fully automatic equipment dedicated to the routine analysis of the milk composition. To enhance the heterogeneity, cow and buffalo bulk milk was collected for a period of over three years from different dairy farms. A total of 119 samples were used for the analysis to generate 17 different concentrations of buffalo-cow milk mixtures. This procedure was used to enhance variability and to properly randomize the trials. The obtained calibration model showed an ≥ 0.99 ( cal. = 0.99861; root mean square error of cross-validation [RMSEC] = 2.04; val. = 0.99803; root mean square error of prediction [RMSEP] = 2.84; root mean square error of cross-validation [RMSECV] = 2.44) suggesting that this method could be successfully applied in the routine analysis of buffalo milk composition, providing rapid screening for possible adulteration with cow's milk at no additional cost.
在意大利，水牛奶酪是一种销量和消费量都很大的乳制品。用水牛牛奶掺假，混入更便宜且更容易获得的其他物种的牛奶，这种情况非常常见。在本研究中，傅里叶变换红外光谱法（FTIR）结合偏最小二乘（PLS）回归的多变量分析，通过使用一台专门用于牛奶成分常规分析的全自动设备，对水牛奶掺假牛奶进行定量检测。为了增加异质性，在三年多的时间里，从不同奶牛场收集了大量的牛奶和水牛奶。总共119个样品用于分析，以生成17种不同浓度的水牛 - 牛奶混合物。此程序用于增加变异性并适当地随机化试验。所获得的校准模型显示相关系数≥0.99（校准 = 0.99861；交叉验证均方根误差[RMSEC] = 2.04；验证 = 0.99803；预测均方根误差[RMSEP] = 2.84；交叉验证均方根误差[RMSECV] = 2.44），这表明该方法可成功应用于水牛奶成分的常规分析，无需额外成本即可快速筛查是否可能掺假牛奶。
