Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Ain Shams University, Cairo, Egypt.
Orthopedic Surgery and Traumatology Department, Faculty of Medicine, Ain Shams University, Cairo, Egypt.
J Cell Biochem. 2019 Sep;120(9):15518-15526. doi: 10.1002/jcb.28818. Epub 2019 May 5.
Osteoporosis is a bone disease characterized by chronic pain and recurrent fractures. Osterix is a transcription factor regulating bone formation. miR-214 was found to have a role in skeletogenesis. Our goal was to investigate the possible role of miR-214 in primary osteoporosis through osterix. Their expression was determined in bone samples obtained from primary osteoporotic patients (n = 26) and age- and sex-matched controls (n = 14). Additionally, their expression was correlated to the laboratory and clinical parameters of the study participants. Differential expression of osterix and miR-214 was detected in the osteoporotic group compared to controls. While miR-214 was significantly higher, osterix was significantly lower. In primary osteoporotic patients, relative quantification value of osterix was positively correlated with sex, body mass index, and ionized calcium and negatively correlated with miR-214 and C-reactive protein. Thus, the role of miR-214 in primary osteoporosis could be through inhibiting osterix expression in bones and therefore both miR-214 and osterix could be targets for future therapeutic intervention.
骨质疏松症是一种以慢性疼痛和反复骨折为特征的骨骼疾病。Osterix 是一种调节骨骼形成的转录因子。miR-214 在骨骼发生中具有作用。我们的目标是通过 Osterix 研究 miR-214 在原发性骨质疏松症中的可能作用。从原发性骨质疏松症患者（n=26）和年龄及性别匹配的对照组（n=14）获得的骨样本中确定了它们的表达。此外，还将它们的表达与研究参与者的实验室和临床参数相关联。与对照组相比，骨质疏松组中检测到 Osterix 和 miR-214 的差异表达。miR-214 明显升高，而 Osterix 明显降低。在原发性骨质疏松症患者中，osterix 的相对定量值与性别、体重指数和离子钙呈正相关，与 miR-214 和 C 反应蛋白呈负相关。因此，miR-214 在原发性骨质疏松症中的作用可能是通过抑制骨骼中 Osterix 的表达，因此 miR-214 和 Osterix 都可能成为未来治疗干预的靶点。
