Wang Qiang, Wang Yuan, Niu Wen-Min, Yang Xiao-Hang, Liu Zhi-Bin
College of Acupuncture and Moxibustion, Shaanxi University of Chinese Medicine, Xianyang 712046, Shaanxi Province, China; Shaanxi Key Laboratory of Combined Acupuncture and Medicine, Xianyang 712046, Shaanxi Province, China.
Zhen Ci Yan Jiu. 2019 Jul 25;44(7):501-5. doi: 10.13702/j.1000-0607.180023.
To observe the effect of electroacupuncture (EA) on expression of 5-HT receptor (5-HTR) and c-fos proteins in the hypothalamus and colon tissues in functional diarrhea (FD) rats so as to explore its underlying mechanisms in improving intestinal function via brain-gut axis.
A total of 40 SD rats were randomized into blank control, model, EA Tianshu (ST25)-Dachangshu (BL25, ST25-BL25) and EA Quchi (LI11)- Shangjuxu (ST37, LI11-ST37) groups. The FD model was established by gavage of Folium Sennae (10 mg/kg) and constraint immobilization once daily for 29 days. EA (10 Hz/50 Hz, 1.5 mA) was applied to bilateral ST25 and BL25 in EA ST25-BL25 group, and bilateral LI11 and ST37 in EA LI11-ST37 group for 20 min, once daily for successive 10 days. The expression of 5-HTR and c-fos protein in the hypothalamus and colon tissues was determined by using Western blot. The state of stool was recorded for calculating the loose stool rate and diarrhea index.
After modeling, the loose stool rate, diarrhea index and expression levels of 5-HTR and c-fos proteins in the colon and hypothalamus tissues were obviously increased in the model group in contrast with the blank control group(<0.01，<0.05). Following EA intervention, the loose stool rate and diarrhea index, the expression levels of 5-HTR and c-fos proteins in the hypothalamus and colon in the EA ST25-BL25 group, and the expression of 5-HTR in the colon in the EA LI11-ST37 group were significantly down-regulated relevant to the model group (<0.01，<0.05). No significant changes were found in loose stool rate and diarrhea index，and the expression levels of hypothalamic 5-HTR and hypothalamic and colonic c-fos proteins in the EA LI11-ST37 group (>0.05). The expression levels of 5-HTR protein in the hypothalamus and c-fos protein in the hypothalamus and colon in the EA LI11-ST37 group were significantly up-regulated relevant to the EA ST25-BL25 group (<0.05).
EA of ST25-BL25 can down-regulate expression of 5-HTR and c-fos proteins in the hypothalamus and colon tissue in FD rats, which may contribute to its function in improving symptoms of FD possibly via brain-gut axis.
观察电针（EA）对功能性腹泻（FD）大鼠下丘脑及结肠组织中5-羟色胺受体（5-HTR）和c-fos蛋白表达的影响，以探讨其通过脑-肠轴改善肠道功能的潜在机制。
将40只SD大鼠随机分为空白对照组、模型组、电针天枢（ST25）-大肠俞（BL25，ST25-BL25）组和电针曲池（LI11）-上巨虚（ST37，LI11-ST37）组。通过每日灌胃番泻叶（10mg/kg）并束缚固定建立FD模型，持续29天。电针ST25-BL25组将电针（10Hz/50Hz，1.5mA）施加于双侧ST25和BL25，电针LI11-ST37组施加于双侧LI11和ST37，持续20分钟，每日1次，连续10天。采用蛋白质免疫印迹法检测下丘脑和结肠组织中5-HTR和c-fos蛋白的表达。记录粪便状态以计算稀便率和腹泻指数。
造模后，模型组稀便率、腹泻指数以及结肠和下丘脑组织中5-HTR和c-fos蛋白的表达水平与空白对照组相比明显升高（<0.01，<0.05）。电针干预后，电针ST25-BL25组的稀便率、腹泻指数、下丘脑和结肠中5-HTR和c-fos蛋白的表达水平，以及电针LI11-ST37组结肠中5-HTR的表达与模型组相比均显著下调（<0.01，<0.05）。电针LI11-ST37组的稀便率、腹泻指数以及下丘脑5-HTR和下丘脑及结肠c-fos蛋白的表达水平无明显变化（>0.05）。电针LI11-ST37组下丘脑5-HTR蛋白以及下丘脑和结肠中c-fos蛋白的表达水平与电针ST25-BL25组相比显著上调（<0.05）。
ST25-BL25电针可下调FD大鼠下丘脑和结肠组织中5-HTR和c-fos蛋白的表达，这可能是其通过脑-肠轴改善FD症状的作用机制。
