Wang Zheng-Wen, Chen Bi-Wei, Chen Shao-Zong, Zhang Li-Li, Sun Hong-Wei, Chang Xiao-Li
Institute of Acupuncture and Moxibustion, Shandong University of Traditional Chinese Medicine, Jinan 250300, China.
College of Acupuncture-Moxibustion and Tuina, Shandong University of Traditional Chinese Medicine, Jinan 250355.
Zhen Ci Yan Jiu. 2023 Feb 25;48(2):165-71. doi: 10.13702/j.1000-0607.20211324.
To study whether electroacupuncture (EA) of "Zusanli" (ST36) combined with "Tianshu" (ST25) has a synergistic effect in regulating the colonic function and autonomic nerve balance in rats with irritable bowel syndrome (IBS).
Male Wistar rats were randomly divided into control, model, EA-ST36, and EA-ST36+ST25 groups, with 14 rats in each group. The IBS model was established by using water avoidance stress method. The visceral hypersensitivity was measured using the abdominal wall retraction reflex (AWR). The rectus abdominis electromyogram (EMG), intestinal electrical activity, and electrocardiogram (ECG) were recorded using a PowerLab data acquisition and analysis system. The contents of serum cAMP and cGMP were determined by ELISA, the expression levels of colonic tyrosine hydroxylase (TH) and choline acetyl-transferase (ChAT) proteins were determined by immunofluorescence staining and Western blot, respectively.
Compared with the control group, the model group had an evident increase in the levels of AWR, LF, LF/HF, ChAT protein expression, cAMP and cGMP contents and cAMP/cGMP ratio (<0.001, <0.05), and a marked decrease in the levels of HF, frequency of slow waves of intestinal EMG, visceral pain threshold (PT), immunoactivity and expression of TH protein (<0.05, <0.001). In contrast to the model group, the levels of AWR, LF, LF/HF, ChAT protein expression and immunoactivity, cAMP and cGMP contents and ratio of cAMP/cGMP were significantly reduced (<0.001, <0.05, <0.01), whereas the levels of frequency of slow waves of intestinal EMG, PT, and the immunoactivity and expression of TH were considerably increased (<0.001, <0.05) in both EA-ST36 and EA-ST36+ST25 groups.
EA of both ST36 and ST36+ST25 can relieve visceral pain, and reduce sympathetic activity to improve autonomic nerve balance, but without apparent synergistic effect between EA-ST36 and EA-ST25 in rats with IBS.
研究电针“足三里”（ST36）联合“天枢”（ST25）对肠易激综合征（IBS）大鼠结肠功能及自主神经平衡是否具有协同调节作用。
将雄性Wistar大鼠随机分为对照组、模型组、电针ST36组和电针ST36+ST25组，每组14只。采用水回避应激法建立IBS模型。采用腹壁回撤反射（AWR）测定内脏高敏感性。使用PowerLab数据采集与分析系统记录腹直肌肌电图（EMG）、肠电活动和心电图（ECG）。采用ELISA法测定血清cAMP和cGMP含量，分别采用免疫荧光染色和蛋白质免疫印迹法测定结肠酪氨酸羟化酶（TH）和胆碱乙酰转移酶（ChAT）蛋白的表达水平。
与对照组相比，模型组AWR、低频（LF）、LF/高频（HF）、ChAT蛋白表达、cAMP和cGMP含量及cAMP/cGMP比值均明显升高（<0.001，<0.05），HF、肠EMG慢波频率、内脏痛阈（PT）、TH蛋白免疫活性及表达水平均明显降低（<0.05，<0.001）。与模型组相比，电针ST36组和电针ST36+ST25组AWR、LF、LF/HF、ChAT蛋白表达及免疫活性、cAMP和cGMP含量及cAMP/cGMP比值均显著降低（<0.001，<0.05，<0.01），而肠EMG慢波频率、PT以及TH免疫活性和表达水平均显著升高（<0.001，<0.05）。
电针ST36及ST36+ST25均能缓解内脏疼痛，降低交感神经活性以改善自主神经平衡，但电针ST36与ST25联合应用对IBS大鼠无明显协同作用。
