Benc Michal, Fulka Josef Jr, Strejček František, Morovič Martin, Murín Matej, Martínková Stanislava, Jettmarová Dominika, Laurinčík Jozef
Institute of Animal Science, Prague, Czech Republic.
Int J Dev Biol. 2019;63(3-4-5):253-258. doi: 10.1387/ijdb.190002mb.
The oocyte GV/GVs (germinal vesicle/germinal vesicles) and zygot PN/PNs (pronucleus/pronuclei) of some mammals contain clearly visible nucleoli which exhibit an atypical morphological structure. These nucleoli (NCLs) can be relatively easily manipulated, i.e. removed from GVs/PNs or eventually transferred into another oocyte/zygote. Thus, with the help of micromanipulation techniques it was possible to uncover the real function(s) they play in processes of oocyte maturation and early embryonic development. The purpose of our review is to describe briefly the micromanipulation techniques that can be used for oocyte/zygote nucleoli manipulation. Moreover, we present some examples of results that were obtained in nucleolus manipulation experiments.
一些哺乳动物的卵母细胞生发泡（GV/生发泡）和受精卵原核（PN/原核）含有清晰可见的核仁，这些核仁呈现出非典型的形态结构。这些核仁（NCL）相对容易操作，即从GV/PN中移除，或者最终转移到另一个卵母细胞/受精卵中。因此，借助显微操作技术，有可能揭示它们在卵母细胞成熟和早期胚胎发育过程中所起的真正作用。我们综述的目的是简要描述可用于卵母细胞/受精卵核仁操作的显微操作技术。此外，我们还展示了一些在核仁操作实验中获得的结果示例。
