White D J, Nancollas G H
Calcif Tissue Int. 1987 Feb;40(2):79-84. doi: 10.1007/BF02555709.
A constant composition method has been used to compare the effects of triamterene renal stone material, synthetic triamterene precipitates, and soluble triamterene on the nucleation and crystallization kinetics of calcium oxalate in aqueous solution in vitro. Crystallization studies have been carried out with the concentrations of calcium and oxalate ions maintained constant by the potentiometrically controlled addition of concentrated reagent solutions containing these ions. Triamterene renal stones were found to be much less effective than synthetic triamterene towards promoting the nucleation and crystallization of calcium oxalate from supersaturated solution. Renal stones composed of triamterene and matrix did not significantly enhance the deposition of calcium oxalate compared to nonseeded controls. The triamterene stones were also found to be ineffective in promoting calcium oxalate crystallization compared to other precipitates thought to be involved in the etiology of stone disease such as calcium hydroxyapatite. For stones of mixed triamterene/calcium oxalate composition, the enhancement of the nucleation and crystallization of calcium oxalate was directly related to the calcium oxalate content of the stone seed material. The presence of soluble triamterene or its metabolites in solution did not influence the crystallization kinetics of pure calcium oxalate seed materials. The results of this study indicate that triamterene in stones does not significantly contribute to further stone development through the enhancement of calcium oxalate crystallization processes.
采用恒组成法，在体外比较氨苯蝶啶肾结石材料、合成氨苯蝶啶沉淀物和可溶性氨苯蝶啶对水溶液中草酸钙成核和结晶动力学的影响。通过电位控制添加含有这些离子的浓试剂溶液，使钙和草酸根离子浓度保持恒定，进行了结晶研究。发现氨苯蝶啶肾结石在促进草酸钙从过饱和溶液中结晶和成核方面比合成氨苯蝶啶的效果差得多。与未接种的对照相比，由氨苯蝶啶和基质组成的肾结石并没有显著增强草酸钙的沉积。与其他被认为与结石病病因有关的沉淀物（如羟基磷灰石钙）相比，氨苯蝶啶结石在促进草酸钙结晶方面也无效。对于氨苯蝶啶/草酸钙混合组成的结石，草酸钙成核和结晶的增强与结石籽晶材料中的草酸钙含量直接相关。溶液中可溶性氨苯蝶啶或其代谢物的存在不影响纯草酸钙籽晶材料的结晶动力学。本研究结果表明，结石中的氨苯蝶啶不会通过增强草酸钙结晶过程而显著促进结石的进一步发展。
